MetaCyc Pathway: ammonia assimilation cycle III in Escherichia coli BW25113
| Reactions and Genes | L-Aspartic Acid (N) |
|---|---|
| remove | |
| Glutamate synthase (NADPH): L-glutamine + H2O→ammonia + L-glutamate + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7) | |
| b0032 (carA) | -5.2 |
| b0033 (carB) | -4.6 |
| b0485 (ybaS) | -0.0 |
| b0674 (asnB) | -1.7 |
| b1263 (trpD) | -2.7 |
| b1264 (trpE) | -2.2 |
| b1524 (yneH) | -0.9 |
| b1812 (pabB) | -0.4 |
| b2468 (aegA) | +0.1 |
| b2507 (guaA) | N.D. |
| b2611 (ypjD) | -0.4 |
| b2780 (pyrG) | N.D. |
| b2887 (ygfT) | +0.2 |
| b3212 (gltB) | +0.2 |
| b3213 (gltD) | +0.2 |
| b3360 (pabA) | +0.1 |
| Glutamate synthase (NADPH) (in reverse): ammonia + 2-oxoglutarate + NADPH + 2 H+→L-glutamate + NADP+ + H2O (EC 1.4.1.13; 1.4.1.14) | |
| b2468 (aegA) | +0.1 |
| b2611 (ypjD) | -0.4 |
| b2887 (ygfT) | +0.2 |
| b3212 (gltB) | +0.2 |
| b3213 (gltD) | +0.2 |
| Glutamate--ammonia ligase: L-glutamate + ammonium + ATP→L-glutamine + ADP + H+ + phosphate (EC 6.3.1.2) | |
| b3870 (glnA) | -2.5 |
Links:
- Pathway details at MetaCyc
- Fitness data for 17 genes
- All MetaCyc pathways for Escherichia coli BW25113
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information