MetaCyc Pathway: TCA cycle V (2-oxoglutarate synthase) in Escherichia coli BW25113

Reactions and Genes	casaminos (C)
2-oxoglutarate synthase: 2-oxoglutarate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→succinyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.3)
No genes
Succinate--CoA ligase (ADP-forming) (in reverse): succinyl-CoA + ADP + phosphate→succinate + ATP + coenzyme A (EC 6.2.1.5)
b0728 (sucC)	-4.0
b0729 (sucD)	-4.2
Succinate dehydrogenase (ubiquinone): succinate[in] + an electron-transfer quinone→fumarate[in] + an electron-transfer quinol (EC 1.3.5.1)
No genes
Fumarate hydratase (in reverse): fumarate + H2O→(S)-malate (EC 4.2.1.2)
b1611 (fumC)	+0.3
b1612 (fumA)	-1.8
b1675 (ydhZ)	-0.3
b2929 (yggD)	-0.2
b4122 (fumB)	-0.2
Malate dehydrogenase: (S)-malate + NAD+→oxaloacetate + NADH + H+ (EC 1.1.1.37; 1.1.1.38)
b0801 (ybiC)	-0.1
b1479 (sfcA)	-0.2
b3236 (mdh)	-3.1
Citrate (Si)-synthase: oxaloacetate + acetyl-CoA + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
b0720 (gltA)	-2.3
Aconitate hydratase: citrate→cis-aconitate + H2O (EC 4.2.1.3)
b0118 (acnB)	-2.8
b0771 (ybhJ)	-0.1
b1276 (acnA)	-0.1
Aconitate hydratase: cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
b0118 (acnB)	-2.8
b0771 (ybhJ)	-0.1
b1276 (acnA)	-0.1
Isocitrate dehydrogenase (NADP(+)): D-threo-isocitrate + NADP+→2-oxoglutarate + CO2 + NADPH (EC 1.1.1.42)
b1136 (icd)	N.D.

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information