MetaCyc Pathway: superpathway of L-tryptophan biosynthesis in Escherichia coli BW25113

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Reactions and Genes casaminos (C)
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3-deoxy-7-phosphoheptulonate synthase:
D-erythrose 4-phosphate + phosphoenolpyruvate + H2O→3-deoxy-D-arabino-heptulosonate 7-phosphate + phosphate (EC 2.5.1.54)
b0754 (aroG) +0.1
b1704 (aroH) -2.6
b2601 (aroF) -0.2
3-dehydroquinate synthase:
3-deoxy-D-arabino-heptulosonate 7-phosphate→3-dehydroquinate + phosphate (EC 4.2.3.4)
b3389 (aroB) -3.5
3-dehydroquinate dehydratase:
3-dehydroquinate→3-dehydroshikimate + H2O (EC 4.2.1.10)
b1693 (aroD) -3.3
b2779 (eno) N.D.
Shikimate dehydrogenase (in reverse):
3-dehydroshikimate + NADPH + H+→shikimate + NADP+ (EC 1.1.1.25)
b1692 (ydiB) -0.1
b3281 (aroE) -3.7
Shikimate kinase:
shikimate + ATP→shikimate 3-phosphate + ADP + H+ (EC 2.7.1.71)
b0388 (aroL) +0.0
b3390 (aroK) -1.8
3-phosphoshikimate 1-carboxyvinyltransferase:
shikimate 3-phosphate + phosphoenolpyruvate→5-enolpyruvoyl-shikimate 3-phosphate + phosphate (EC 2.5.1.19)
b0908 (aroA) -2.5
Chorismate synthase:
5-enolpyruvoyl-shikimate 3-phosphate→chorismate + phosphate (EC 4.2.3.5)
b2329 (aroC) -3.5
Anthranilate synthase:
chorismate + L-glutamine→anthranilate + L-glutamate + H+ + pyruvate (EC 4.1.3.27)
b1263 (trpD) -4.5
b1264 (trpE) -4.2
Anthranilate phosphoribosyltransferase (in reverse):
anthranilate + 5-phospho-α-D-ribose 1-diphosphate→N-(5-phosphoribosyl)-anthranilate + diphosphate (EC 2.4.2.18)
b0800 (ybiB) +0.0
b1263 (trpD) -4.5
Phosphoribosylanthranilate isomerase:
N-(5-phosphoribosyl)-anthranilate→1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate (EC 5.3.1.24)
b1262 (trpC) -4.3
Indole-3-glycerol-phosphate synthase:
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H+→(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O (EC 4.1.1.48)
b1262 (trpC) -4.3
Indole-3-glycerol-phosphate lyase:
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate→indole + D-glyceraldehyde 3-phosphate (EC 4.1.2.8; 4.2.1.20)
b1260 (trpA) -4.4
b1261 (trpB) -6.8
indole + L-serine→L-tryptophan + H2O (EC 4.2.1.122; 4.2.1.20)
b1260 (trpA) -4.4
b1261 (trpB) -6.8

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information