MetaCyc Pathway: superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle in Escherichia coli BW25113

Reactions and Genes	casaminos (C)
Diphosphate--fructose-6-phosphate 1-phosphotransferase: β-D-fructofuranose 6-phosphate + diphosphate→β-D-fructofuranose 1,6-bisphosphate + H+ + phosphate (EC 2.7.1.90)
No genes
6-phosphofructokinase: β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H+ (EC 2.7.1.11)
b1723 (pfkB)	+0.1
b3916 (pfkA)	-0.2
Fructose-bisphosphate aldolase: β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)
b2097 (b2097)	+0.1
b2925 (fbaA)	N.D.
Triose-phosphate isomerase (in reverse): glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
b3919 (tpiA)	N.D.
Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)): D-glyceraldehyde 3-phosphate + NADP+ + H2O→3-phospho-D-glycerate + NADPH + 2 H+ (EC 1.2.1.9)
No genes
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating): D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
b1779 (gapA)	N.D.
Phosphoglycerate kinase (in reverse): 3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
b2926 (pgk)	N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
b0755 (gpmA)	+0.0
b3612 (gpmI)	-0.1
b4395 (ytjC)	-0.4
Phosphopyruvate hydratase: 2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
b2779 (eno)	N.D.
Pyruvate kinase (in reverse): phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
b1676 (pykF)	+0.0
b1854 (pykA)	-0.1
pyruvate + a [pyruvate dehydrogenase E2 protein] N6-lipoyl-L-lysine + H+→a [pyruvate dehydrogenase E2 protein] N6-S-acetyldihydrolipoyl-L-lysine + CO2 (EC 1.2.1.104; 1.2.4.1)
b0114 (aceE)	-3.0
a [pyruvate dehydrogenase E2 protein] N6-S-acetyldihydrolipoyl-L-lysine + coenzyme A→acetyl-CoA + a [pyruvate dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine (EC 1.2.1.104; 2.3.1.12)
b0115 (aceF)	N.D.
Citrate (Si)-synthase: oxaloacetate + acetyl-CoA + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
b0720 (gltA)	-2.3
a [pyruvate dehydrogenase E2 protein] N6-dihydrolipoyl-L-lysine + NAD+→a [pyruvate dehydrogenase E2 protein] N6-lipoyl-L-lysine + NADH + H+ (EC 1.2.1.104; 1.8.1.4)
b0116 (lpd)	N.D.
b0304 (ykgC)	-0.1
Aconitate hydratase: citrate→cis-aconitate + H2O (EC 4.2.1.3)
b0118 (acnB)	-2.8
b0771 (ybhJ)	-0.1
b1276 (acnA)	-0.1
Aconitate hydratase: cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
b0118 (acnB)	-2.8
b0771 (ybhJ)	-0.1
b1276 (acnA)	-0.1
Isocitrate--homoisocitrate dehydrogenase: D-threo-isocitrate + NAD+→2-oxoglutarate + CO2 + NADH (EC 1.1.1.286; 1.1.1.41)
No genes
2-oxoglutarate + coenzyme A + NAD+→succinyl-CoA + CO2 + NADH (EC 1.2.1.105)
b0116 (lpd)	N.D.
Succinate--CoA ligase (ADP-forming) (in reverse): succinyl-CoA + ADP + phosphate→succinate + ATP + coenzyme A (EC 6.2.1.5)
b0728 (sucC)	-4.0
b0729 (sucD)	-4.2
Succinate dehydrogenase (ubiquinone): succinate[in] + an electron-transfer quinone→fumarate[in] + an electron-transfer quinol (EC 1.3.5.1)
No genes
Fumarate hydratase (in reverse): fumarate + H2O→(S)-malate (EC 4.2.1.2)
b1611 (fumC)	+0.3
b1612 (fumA)	-1.8
b1675 (ydhZ)	-0.3
b2929 (yggD)	-0.2
b4122 (fumB)	-0.2
Malate dehydrogenase: (S)-malate + NAD+→oxaloacetate + NADH + H+ (EC 1.1.1.37; 1.1.1.38)
b0801 (ybiC)	-0.1
b1479 (sfcA)	-0.2
b3236 (mdh)	-3.1

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information