MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Variovorax sp. OAS795

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Reactions and Genes D-Xylose (C)
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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
ABID97_RS04085 -0.1
ABID97_RS12545 +0.2
ABID97_RS13280 +0.1
ABID97_RS14885 N.D.
ABID97_RS14890 -1.8
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
ABID97_RS14895 N.D.
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
ABID97_RS03710 -0.0
ABID97_RS06870 +1.2
ABID97_RS17500 N.D.
ABID97_RS21205 -7.3
ABID97_RS25645 +0.1
ABID97_RS25870 +0.0
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
ABID97_RS10235 -0.0
ABID97_RS13195 -0.8
ABID97_RS13275 -0.1
ABID97_RS15955 -0.2
ABID97_RS18840 -0.0
ABID97_RS25565 +0.4
ABID97_RS27980 -0.4
ABID97_RS29150 -0.8
ABID97_RS29360 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information