MetaCyc Pathway: tRNA-uridine 2-thiolation (cytoplasmic) in Escherichia coli BW25113

Reactions and Genes	L-Malic (C)
a ubiquitin-related modifier 1 + ATP + H+→a carboxy-adenylated-[ubiquitin-related modifier 1] + diphosphate
No genes
a uridine34 in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O→a carboxymethyluridine34 in tRNA + 5'-deoxyadenosine + coenzyme A + L-methionine + 2 H+ (EC 2.3.1.311)
No genes
Cysteine desulfurase: L-cysteine + an [L-cysteine desulfurase]-L-cysteine→an [L-cysteine desulfurase]-S-sulfanyl-L-cysteine + L-alanine (EC 2.8.1.7)
b1680 (sufS)	-0.1
b2810 (csdA)	+1.1
b2811 (ygdK)	+0.8
b2530 (iscS)	N.D.
a carboxymethyluridine34 in tRNA + S-adenosyl-L-methionine→a 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA + S-adenosyl-L-homocysteine (EC 2.1.1.229)
No genes
an [L-cysteine desulfurase]-S-sulfanyl-L-cysteine + a [3-mercaptopyruvate sulfurtransferase]-L-cysteine→a [3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine + an [L-cysteine desulfurase]-L-cysteine
No genes
a [3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine + a [MOCS3 protein]-L-cysteine→a [MOCS3]-S-sulfanylcysteine + a [3-mercaptopyruvate sulfurtransferase]-L-cysteine
No genes
a carboxy-adenylated-[ubiquitin-related modifier 1] + a [MOCS3]-S-sulfanylcysteine + a reduced two electron carrier→a thiocarboxylated-[ubiquitin-related modifier 1] + AMP + an oxidized electron carrier + a [MOCS3 protein]-L-cysteine + H+
No genes
a 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA + a thiocarboxylated-[ubiquitin-related modifier 1] + ATP + H2O→a ubiquitin-related modifier 1 + a 5-(2-methoxy-2-oxoethyl)2-thiouridine34 in tRNA + AMP + diphosphate + 2 H+
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information