MetaCyc Pathway: tRNA-uridine 2-thiolation and selenation (bacteria) in Escherichia coli BW25113

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Reactions and Genes L-Malic (C)
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Cysteine desulfurase:
L-cysteine + a [TusA]-L-cysteine→a [TusA sulfur-carrier protein]-S-sulfanyl-L-cysteine + L-alanine (EC 2.8.1.7)
b1680 (sufS) -0.2
b2810 (csdA) +1.1
b2811 (ygdK) +1.1
b2530 (iscS) N.D.
a [TusA sulfur-carrier protein]-S-sulfanyl-L-cysteine + a [TusD]-L-cysteine→a [TusD sulfur-carrier protein]-S-sulfanyl-L-cysteine + a [TusA]-L-cysteine
b3470 (yhhP) N.D.
a [TusD sulfur-carrier protein]-S-sulfanyl-L-cysteine + a [TusE sulfur carrier protein]-L-cysteine→a [TusE sulfur carrier protein]-S-sulfanylcysteine + a [TusD]-L-cysteine
b3343 (yheL) N.D.
b3344 (yheM) N.D.
b3345 (yheN) N.D.
a [TusE sulfur carrier protein]-S-sulfanylcysteine + a [tRNA-uridine 2-sulfurtransferase]-L-cysteine→a [tRNA-uridine 2-sulfurtransferase]-S-sulfanyl-L-cysteine + a [TusE sulfur carrier protein]-L-cysteine
b0969 (yccK) N.D.
a [tRNA-uridine 2-sulfurtransferase]-S-sulfanyl-L-cysteine + a uridine34 in tRNA + ATP + a reduced two electron carrier→a 2-thiouridine34 in tRNA + AMP + an oxidized electron carrier + a [tRNA-uridine 2-sulfurtransferase]-L-cysteine + diphosphate + H+ (EC 2.8.1.13)
b1133 (ycfB) N.D.
a 2-thiouridine34 in tRNA + an oxidized electron carrier + glycine + GTP + a 5,10-methylenetetrahydrofolate + H2O→a 5-carboxymethylaminomethyl-2-thiouridine34 in tRNA + a 7,8-dihydrofolate + a reduced two electron carrier + GDP + H+ + phosphate
No genes
a 5-carboxymethylaminomethyl-2-thiouridine34 in tRNA + an oxidized electron carrier + H2O→a 5-(aminomethyl)-2-thiouridine34 in tRNA + a reduced two electron carrier + glyoxylate
b2324 (b2324) +0.3
tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase:
a 5-(aminomethyl)-2-thiouridine34 in tRNA + S-adenosyl-L-methionine→a 5-[(methylamino)methyl]-2-thiouridine34 in tRNA + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.61)
b1133 (ycfB) N.D.
b2324 (b2324) +0.3
a 5-[(methylamino)methyl]-2-thiouridine34 in tRNA + geranyl diphosphate→a 5-methylaminomethyl-2-(S-geranyl)thiouridine34 in tRNA + diphosphate (EC 2.9.1.3)
No genes
a 5-methylaminomethyl-2-(S-geranyl)thiouridine34 in tRNA + selenophosphate→a 5-methylaminomethyl-2-(Se-phospho)selenouridine34 in tRNA + (2E)-3,7-dimethylocta-2,6-diene-1-thiol (EC 2.9.1.3)
No genes
a 5-methylaminomethyl-2-(Se-phospho)selenouridine34 in tRNA + H2O→a 5-methylaminomethyl-2-selenouridine34 in tRNA + phosphate (EC 2.9.1.3)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information