MetaCyc Pathway: (S)-lactate fermentation to propanoate, acetate and hydrogen in Escherichia coli BW25113

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Reactions and Genes succinate (C)
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L-lactate dehydrogenase:
(S)-lactate + NAD+→pyruvate + NADH + H+ (EC 1.1.1.27)
b0306 (ykgE) -0.0
b0307 (ykgF) +0.2
Pyruvate carboxylase:
pyruvate + ATP + hydrogen carbonate→oxaloacetate + ADP + H+ + phosphate (EC 6.4.1.1)
No genes
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
b0823 (ybiW) +0.3
b0824 (ybiY) -0.1
b0902 (pflA) -0.7
b0903 (pflB) -0.6
b2579 (yfiD) -0.1
b3951 (pflD) -0.1
b3952 (pflC) -0.1
b3114 (tdcE) -0.0
formate + H+→H2 + CO2
b2483 (hyfC) +0.2
b2722 (hycD) -0.1
b2723 (hycC) -0.3
b2724 (hycB) -0.0
b4079 (fdhF) -0.1
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
b0801 (ybiC) +0.3
b1479 (sfcA) -2.8
b3236 (mdh) -1.4
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
b2297 (pta) -3.0
b2458 (eutI) +0.1
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
b1849 (purT) -0.1
b2296 (ackA) N.D.
b2460 (eutQ) +0.0
b2461 (eutP) -0.1
b3115 (tdcD) -0.1
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
b1611 (fumC) +0.4
b1612 (fumA) -1.7
b1675 (ydhZ) -0.4
b2929 (yggD) -0.0
b4122 (fumB) -0.0
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
b4151 (frdD) +0.1
b4152 (frdC) +0.3
b4153 (frdB) +0.1
b4154 (frdA) +0.4
succinate + propanoyl-CoA→succinyl-CoA + propanoate (EC 2.8.3.27)
b2920 (ygfH) +0.2
Methylmalonyl-CoA mutase (in reverse):
succinyl-CoA→(R)-methylmalonyl-CoA (EC 5.4.99.2)
b2917 (yliK) +0.0
Methylmalonyl-CoA epimerase:
(R)-methylmalonyl-CoA→(S)-methylmalonyl-CoA (EC 5.1.99.1)
No genes
(S)-methylmalonyl-CoA[in] + Na+[in] + H+[out]→propanoyl-CoA[in] + CO2[in] + Na+[out] (EC 7.2.4.3)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information