MetaCyc Pathway: gluconeogenesis I in Escherichia coli BW25113

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Reactions and Genes succinate (C)
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Malate dehydrogenase (oxaloacetate-decarboxylating):
(S)-malate + NAD+→pyruvate + CO2 + NADH (EC 1.1.1.38)
b1479 (sfcA) -2.8
Malate dehydrogenase:
(S)-malate + NAD+→oxaloacetate + NADH + H+ (EC 1.1.1.37; 1.1.1.38)
b0801 (ybiC) +0.3
b1479 (sfcA) -2.8
b3236 (mdh) -1.4
Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)):
(S)-malate + NADP+→pyruvate + CO2 + NADPH (EC 1.1.1.40)
b2463 (maeB) -1.1
Phosphoenolpyruvate carboxykinase (ATP):
oxaloacetate + ATP→phosphoenolpyruvate + ADP + CO2 (EC 4.1.1.49)
b3403 (pck) -2.0
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2)
b1702 (pps) -0.2
Phosphopyruvate hydratase (in reverse):
phosphoenolpyruvate + H2O→2-phospho-D-glycerate (EC 4.2.1.11)
b2779 (eno) N.D.
2-phospho-D-glycerate→3-phospho-D-glycerate (EC 5.4.2.12)
b0755 (gpmA) -0.1
b3612 (gpmI) -0.0
b4395 (ytjC) -0.6
Phosphoglycerate kinase:
3-phospho-D-glycerate + ATP→3-phospho-D-glyceroyl phosphate + ADP (EC 2.7.2.3)
b2926 (pgk) N.D.
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (in reverse):
3-phospho-D-glyceroyl phosphate + NADH + H+→D-glyceraldehyde 3-phosphate + NAD+ + phosphate (EC 1.2.1.12)
b1779 (gapA) N.D.
Fructose-bisphosphate aldolase (in reverse):
D-glyceraldehyde 3-phosphate + glycerone phosphate→β-D-fructofuranose 1,6-bisphosphate (EC 4.1.2.13)
b2097 (b2097) +0.1
b2925 (fbaA) N.D.
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
b0383 (phoA) +0.3
b0766 (ybhA) -0.1
b2930 (yggF) +0.1
b3925 (glpX) +0.1
b4232 (fbp) -5.4
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
b3919 (tpiA) N.D.
Glucose-6-phosphate isomerase (in reverse):
β-D-fructofuranose 6-phosphate→α-D-glucose 6-phosphate (EC 5.3.1.9)
b2843 (kduI) +0.1
b4025 (pgi) +1.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information