MetaCyc Pathway: mixed acid fermentation in Rhizobium sp. OAE497

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Reactions and Genes Formate (C)
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Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)
No genes
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
ABIE40_RS16145 N.D.
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
No genes
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
ABIE40_RS10450 -0.6
ABIE40_RS18000 N.D.
ABIE40_RS25800 -0.1
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
No genes
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
No genes
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
ABIE40_RS09605 +0.2
ABIE40_RS09610 +0.1
formate + H+→CO2 + H2
No genes
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
ABIE40_RS10540 -0.1
ABIE40_RS10550 +0.1
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
No genes
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
No genes
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
ABIE40_RS18275 N.D.
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
ABIE40_RS09860 -0.1
ABIE40_RS11065 +0.1
ABIE40_RS11375 -4.2
ABIE40_RS12020 -0.2
ABIE40_RS12810 -0.1
ABIE40_RS14500 +0.0
ABIE40_RS22235 -0.1
ABIE40_RS25090 -0.2
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
No genes
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
ABIE40_RS18275 N.D.
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→CO2 + 2-oxoglutarate + NADPH (EC 1.1.1.42)
ABIE40_RS10285 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information