MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Rhizobium sp. OAE497

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Reactions and Genes Formate (C)
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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
ABIE40_RS11490 -1.2
ABIE40_RS11495 -4.5
ABIE40_RS11985 +0.1
ABIE40_RS29635 -0.1
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
ABIE40_RS11360 N.D.
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
ABIE40_RS08005 -4.0
ABIE40_RS13975 -0.2
ABIE40_RS26215 +0.2
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
ABIE40_RS09860 +0.1
ABIE40_RS11065 -0.1
ABIE40_RS11375 -3.7
ABIE40_RS12020 +0.0
ABIE40_RS12810 +0.1
ABIE40_RS14500 +0.1
ABIE40_RS22235 -0.2
ABIE40_RS25090 +0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information