MetaCyc Pathway: superpathway of anaerobic sucrose degradation in Escherichia coli BW25113

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Reactions and Genes D,L-Lactate (C)
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Sucrose synthase (in reverse):
sucrose + H+ + UDP→β-D-fructofuranose + UDP-α-D-glucose (EC 2.4.1.13)
No genes
Fructokinase:
β-D-fructofuranose + ATP→β-D-fructofuranose 6-phosphate + ADP + H+ (EC 2.7.1.4)
b0394 (yajF) -0.2
b3883 (yihV) +0.0
UTP-monosaccharide-1-phosphate uridylyltransferase (in reverse):
UDP-α-D-glucose + diphosphate→α-D-glucopyranose 1-phosphate + H+ + UTP (EC 2.7.7.64; 2.7.7.9)
b1236 (galU) +0.1
b2042 (galF) +0.1
6-phosphofructokinase:
β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H+ (EC 2.7.1.11)
b1723 (pfkB) +0.1
b3916 (pfkA) -0.5
Phosphoglucomutase:
α-D-glucopyranose 1-phosphate→D-glucopyranose 6-phosphate (EC 5.4.2.2)
b0688 (pgm) -0.8
b3176 (glmM) N.D.
Fructose-bisphosphate aldolase:
β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)
b2097 (b2097) +0.2
b2925 (fbaA) N.D.
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
b0383 (phoA) +0.3
b0766 (ybhA) +0.3
b2930 (yggF) +0.1
b3925 (glpX) +0.1
b4232 (fbp) -5.2
Glucose-6-phosphate isomerase:
α-D-glucose 6-phosphate→β-D-fructofuranose 6-phosphate (EC 5.3.1.9)
b2843 (kduI) +0.1
b4025 (pgi) +0.1
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
b3919 (tpiA) N.D.
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
b1779 (gapA) N.D.
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
b2926 (pgk) N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
b0755 (gpmA) -0.3
b3612 (gpmI) -0.1
b4395 (ytjC) -0.3
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)
b0755 (gpmA) -0.3
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
b2779 (eno) N.D.
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
b1676 (pykF) -0.1
b1854 (pykA) +0.1
L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(S)-lactate + NAD+ (EC 1.1.1.27)
b0306 (ykgE) +0.0
b0307 (ykgF) +0.3
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2)
b1702 (pps) -4.3
Pyruvate decarboxylase:
pyruvate + H+→acetaldehyde + CO2 (EC 4.1.1.1)
No genes
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
b0325 (yahK) +0.2
b0356 (frmA) +0.0
b1241 (adhE) +0.2
b1478 (adhP) +0.0
b3253 (yhdH) +0.0
b4269 (yjgB) -0.0
b3589 (yiaY) +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information