MetaCyc Pathway: superpathway of fermentation (Chlamydomonas reinhardtii) in Escherichia coli BW25113

Reactions and Genes	D-Lactate (C)
Formate C-acetyltransferase (in reverse): pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
b0823 (ybiW)	+0.1
b0824 (ybiY)	-0.0
b0902 (pflA)	-0.2
b0903 (pflB)	-0.1
b2579 (yfiD)	-0.2
b3951 (pflD)	-0.0
b3952 (pflC)	-0.0
b3114 (tdcE)	+0.0
Pyruvate decarboxylase: pyruvate + H+→acetaldehyde + CO2 (EC 4.1.1.1)
No genes
Acetaldehyde dehydrogenase (acetylating) (in reverse): acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
b0351 (mhpF)	+0.1
b1241 (adhE)	+0.6
b2455 (eutE)	-0.2
Pyruvate synthase: pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
b1378 (ydbK)	+0.0
Alcohol dehydrogenase (in reverse): acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
b0325 (yahK)	+0.1
b0356 (frmA)	+0.1
b1241 (adhE)	+0.6
b1478 (adhP)	-0.1
b3253 (yhdH)	-0.1
b4269 (yjgB)	-0.1
b3589 (yiaY)	+0.1
D-lactate dehydrogenase (in reverse): pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
b1380 (ldhA)	-0.2
b2133 (dld)	-1.3
Ferredoxin hydrogenase (in reverse): 2 a reduced ferredoxin [iron-sulfur] cluster + 2 H+→H2 + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.12.7.2)
b2483 (hyfC)	-0.1
b2719 (hycG)	-0.1
b2720 (hycF)	-0.1
b2721 (hycE)	+0.0
b2722 (hycD)	+0.2
b2723 (hycC)	-0.1
b2724 (hycB)	-0.0
Phosphate acetyltransferase: acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
b2297 (pta)	-2.4
b2458 (eutI)	+0.1
Acetate kinase (in reverse): acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
b1849 (purT)	+0.1
b2296 (ackA)	N.D.
b2460 (eutQ)	+0.2
b2461 (eutP)	+0.0
b3115 (tdcD)	-0.1

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information