MetaCyc Pathway: ceramide degradation by α-oxidation in Pseudomonas fluorescens GW456-L13

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Reactions and Genes Putrescine (C)
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a phytoceramide + 2 a ferrocytochrome b5 + dioxygen + 2 H+→a (2'R)-2'-hydroxy-phytoceramide + 2 a ferricytochrome b5 + H2O (EC 1.14.18.6)
No genes
Ceramidase:
a (2'R)-2'-hydroxy-phytoceramide + H2O→a (2R)-2-hydroxy even numbered straight chain 2,3,4-saturated fatty acid + a phytosphingosine
(EC 3.5.1.23)
No genes
a (2R)-2-hydroxy even numbered straight chain 2,3,4-saturated fatty acid + ATP + coenzyme A→a (R)-2-hydroxy even numbered straight chain 2,3,4-saturated fatty acyl CoA + AMP + diphosphate
No genes
a (R)-2-hydroxy even numbered straight chain 2,3,4-saturated fatty acyl CoA→formyl-CoA + an odd numbered straight chain 2,3,4-saturated fatty aldehyde (EC 4.1.2.63)
No genes
Formyl-CoA hydrolase:
formyl-CoA + H2O→formate + coenzyme A + H+
(EC 3.1.2.10)
No genes
Aldehyde dehydrogenase (NAD(+)):
an odd numbered straight chain 2,3,4-saturated fatty aldehyde + NAD+ + H2O→an odd numbered straight chain 2,3,4-saturated fatty acid + NADH + 2 H+
(EC 1.2.1.3)
PfGW456L13_127 -0.1
PfGW456L13_1342 -0.5
PfGW456L13_1397 +0.1
PfGW456L13_1795 +0.7
PfGW456L13_2360 -0.2
PfGW456L13_2419 -0.2
PfGW456L13_2690 +0.1
PfGW456L13_2713 +0.1
PfGW456L13_3141 -0.4
PfGW456L13_3205 -0.2
PfGW456L13_3440 +0.4
PfGW456L13_3517 -1.7
PfGW456L13_3604 -0.5
PfGW456L13_3737 +0.1
PfGW456L13_5044 +0.1
PfGW456L13_805 -2.8
PfGW456L13_985 -0.2
Long-chain-fatty-acid--CoA ligase:
an odd numbered straight chain 2,3,4-saturated fatty acid + ATP + coenzyme A→an odd numbered straight chain 2,3,4-saturated fatty acyl CoA + AMP + diphosphate
(EC 6.2.1.3)
PfGW456L13_1910 -0.0
PfGW456L13_1911 -0.6
PfGW456L13_2521 +0.2
PfGW456L13_2593 -0.0
PfGW456L13_4159 +0.3

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information