MetaCyc Pathway: (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) in Variovorax sp. OAS795

Reactions and Genes	Tween 20 (C)
Acetyl-CoA C-acyltransferase: 2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
ABID97_RS04675	+0.1
ABID97_RS05875	N.D.
ABID97_RS06005	+0.0
ABID97_RS10975	+0.1
ABID97_RS11335	-0.2
ABID97_RS11380	-0.0
ABID97_RS12420	N.D.
ABID97_RS15750	-0.3
ABID97_RS18615	+0.1
ABID97_RS18775	+0.2
ABID97_RS20240	+0.0
ABID97_RS24190	+0.2
ABID97_RS25195	-0.4
ABID97_RS26575	N.D.
ABID97_RS27835	-0.6
ABID97_RS28230	+0.8
ABID97_RS28270	-0.4
ABID97_RS28430	-0.2
3-hydroxyacyl-CoA dehydrogenase (in reverse): acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
ABID97_RS00810	N.D.
ABID97_RS03030	+0.2
ABID97_RS05865	-1.4
ABID97_RS06010	-0.2
ABID97_RS08805	+0.3
ABID97_RS10560	-0.6
ABID97_RS15740	-0.5
ABID97_RS17005	-0.1
ABID97_RS18595	N.D.
Acetoacetyl-CoA reductase (in reverse): acetoacetyl-CoA + NADPH + H+→(3R)-3-hydroxybutanoyl-CoA + NADP+ (EC 1.1.1.36)
ABID97_RS12460	+1.8
ABID97_RS26580	-0.1
(S)-3-hydroxybutanoyl-CoA + H2O→(S)-3-hydroxybutanoate + coenzyme A + H+
ABID97_RS04285	+0.1
(3R)-3-hydroxybutanoyl-CoA + H2O→(R)-3-hydroxybutanoate + coenzyme A + H+
ABID97_RS04285	+0.1

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information