| Reactions and Genes | Tween 20 (C) |
|---|
| remove |
3-beta-hydroxy-Delta(5)-steroid dehydrogenase:
cholesterol + NAD+→cholest-5-en-3-one + NADH + H+ (EC 1.1.1.145) |
| No genes |
Steroid Delta-isomerase:
cholest-5-en-3-one→cholest-4-en-3-one (EC 5.3.3.1) |
| No genes |
| cholest-4-en-3-one + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(25S)-26-hydroxycholest-4-en-3-one + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.14.15.29) |
| No genes |
| cholest-4-en-3-one + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(25R)-26-hydroxycholest-4-en-3-one + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.14.15.28) |
| No genes |
| (25S)-26-hydroxycholest-4-en-3-one + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(25S)-26-oxocholest-4-en-3-one + 2 an oxidized ferredoxin [iron-sulfur] cluster + 2 H2O (EC 1.14.15.29) |
| No genes |
| (25R)-26-hydroxycholest-4-en-3-one + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(25R)-26-oxocholest-4-en-3-one + 2 an oxidized ferredoxin [iron-sulfur] cluster + 2 H2O (EC 1.14.15.28) |
| No genes |
| (25S)-26-oxocholest-4-en-3-one + dioxygen + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(25S)-3-oxocholest-4-en-26-oate + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.14.15.29) |
| No genes |
| (25R)-26-oxocholest-4-en-3-one + dioxygen + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(25R)-3-oxocholest-4-en-26-oate + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.14.15.28) |
| No genes |
| (25S)-3-oxocholest-4-en-26-oate + ATP + coenzyme A→(25S)-3-oxocholest-4-en-26-oyl-CoA + AMP + diphosphate (EC 6.2.1.42) |
| No genes |
| (25R)-3-oxocholest-4-en-26-oate + ATP + coenzyme A→(25R)-3-oxocholest-4-en-26-oyl-CoA + AMP + diphosphate (EC 6.2.1.42) |
| No genes |
Alpha-methylacyl-CoA racemase (in reverse):
(25R)-3-oxocholest-4-en-26-oyl-CoA→(25S)-3-oxocholest-4-en-26-oyl-CoA (EC 5.1.99.4) |
| ABID97_RS18500 | -0.2 |
| ABID97_RS28195 | -0.0 |
| (25S)-3-oxocholest-4-en-26-oyl-CoA + an oxidized electron carrier→3-oxocholest-4,24-dien-26-oyl-CoA + a reduced two electron carrier |
| No genes |
| 3-oxocholest-4,24-dien-26-oyl-CoA + H2O→24-hydroxy-3-oxocholest-4-en-26-oyl-CoA |
| No genes |
3-hydroxyacyl-CoA dehydrogenase:
24-hydroxy-3-oxocholest-4-en-26-oyl-CoA + NAD+→3,24-dioxocholest-4-en-26-oyl-CoA + NADH + H+ (EC 1.1.1.35) |
| ABID97_RS03030 | +0.2 |
| ABID97_RS05865 | -1.4 |
| ABID97_RS06010 | -0.2 |
| ABID97_RS08805 | +0.3 |
| ABID97_RS10560 | -0.6 |
| ABID97_RS15740 | -0.5 |
| ABID97_RS17005 | -0.1 |
| ABID97_RS18595 | N.D. |
Propanoyl-CoA C-acyltransferase (in reverse):
3,24-dioxocholest-4-en-26-oyl-CoA + coenzyme A→3-oxochol-4-en-24-oyl-CoA + propanoyl-CoA (EC 2.3.1.176) |
| No genes |
| 3-oxochol-4-en-24-oyl-CoA + an oxidized electron carrier→3-oxochol-4,22-dien-24-oyl-CoA + a reduced two electron carrier |
| No genes |
| 3-oxochol-4,22-dien-24-oyl-CoA + H2O→22-hydroxy-3-oxochol-4-en-24-oyl-CoA |
| No genes |
| 22-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+→3,22-dioxochol-4-en-24-oyl-CoA + NADH + H+ |
| No genes |
Acetyl-CoA C-acyltransferase (in reverse):
3,22-dioxochol-4-en-24-oyl-CoA + coenzyme A→acetyl-CoA + 3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA (EC 2.3.1.16) |
| ABID97_RS04675 | +0.1 |
| ABID97_RS05875 | N.D. |
| ABID97_RS06005 | +0.0 |
| ABID97_RS10975 | +0.1 |
| ABID97_RS11335 | -0.2 |
| ABID97_RS11380 | -0.0 |
| ABID97_RS12420 | N.D. |
| ABID97_RS15750 | -0.3 |
| ABID97_RS18615 | +0.1 |
| ABID97_RS18775 | +0.2 |
| ABID97_RS20240 | +0.0 |
| ABID97_RS24190 | +0.2 |
| ABID97_RS25195 | -0.4 |
| ABID97_RS26575 | N.D. |
| ABID97_RS27835 | -0.6 |
| ABID97_RS28230 | +0.8 |
| ABID97_RS28270 | -0.4 |
| ABID97_RS28430 | -0.2 |
| 3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA + an oxidized electron carrier→3-oxo-23,24-bisnorchol-4,17(20)-dien-22-oyl-CoA + a reduced two electron carrier |
| No genes |
| 3-oxo-23,24-bisnorchol-4,17(20)-dien-22-oyl-CoA + H2O→17-hydroxy-3-oxo-4-pregnane-20-carboxyl-CoA |
| No genes |
| 17-hydroxy-3-oxo-4-pregnane-20-carboxyl-CoA→propanoyl-CoA + androstenedione |
| No genes |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information