MetaCyc Pathway: L-isoleucine degradation I in Variovorax sp. OAS795

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Reactions and Genes 4-Hydroxybenzoic Acid (C)
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Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)
ABID97_RS03790 -2.4
2-oxoisovalerate dehydrogenase (acylating):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + NAD+→(2S)-2-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
No genes
(E)-2-methylcrotonoyl-CoA + H2O→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (EC 4.2.1.150)
ABID97_RS00815 +0.1
ABID97_RS01435 +0.1
ABID97_RS02880 +0.2
ABID97_RS04670 +0.0
ABID97_RS05865 -1.6
ABID97_RS05900 +0.0
ABID97_RS05990 -0.1
ABID97_RS06010 +0.3
ABID97_RS07755 +0.2
ABID97_RS10620 N.D.
ABID97_RS15740 -0.3
ABID97_RS17010 +0.0
ABID97_RS17470 +0.2
ABID97_RS18490 -0.2
ABID97_RS18600 +0.2
ABID97_RS18605 N.D.
ABID97_RS18635 -0.8
ABID97_RS20990 +0.5
ABID97_RS22770 +0.0
ABID97_RS24015 +0.4
ABID97_RS24805 +1.5
ABID97_RS26735 N.D.
ABID97_RS27490 +0.1
ABID97_RS27535 +0.6
ABID97_RS27555 -0.2
ABID97_RS27620 N.D.
ABID97_RS28260 N.D.
ABID97_RS28410 N.D.
ABID97_RS29050 -0.3
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+→2-methylacetoacetyl-CoA + NADH + H+ (EC 1.1.1.178)
ABID97_RS03030 +0.2
ABID97_RS05865 -1.6
ABID97_RS06010 +0.3
ABID97_RS10560 -0.0
ABID97_RS15740 -0.3
ABID97_RS17005 -0.5
ABID97_RS18595 N.D.
ABID97_RS24025 -0.4
Acetyl-CoA C-acyltransferase (in reverse):
2-methylacetoacetyl-CoA + coenzyme A→acetyl-CoA + propanoyl-CoA (EC 2.3.1.16)
ABID97_RS04675 +0.2
ABID97_RS05875 N.D.
ABID97_RS06005 +0.4
ABID97_RS10975 +0.0
ABID97_RS11335 +0.8
ABID97_RS11380 +0.1
ABID97_RS12420 N.D.
ABID97_RS15750 +0.1
ABID97_RS18615 -0.1
ABID97_RS18775 +0.4
ABID97_RS20240 -0.1
ABID97_RS24190 -0.2
ABID97_RS25195 +0.5
ABID97_RS26575 N.D.
ABID97_RS27835 +0.0
ABID97_RS28230 -0.4
ABID97_RS28270 -1.0
ABID97_RS28430 -0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information