MetaCyc Pathway: biotin biosynthesis from 8-amino-7-oxononanoate I in Escherichia coli BW25113

Reactions and Genes	a-Ketoglutaric (C)
Adenosylmethionine--8-amino-7-oxononanoate transaminase: (8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine→(7R,8S)-diaminononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate (EC 2.6.1.62)
b0774 (bioA)	-0.7
Dethiobiotin synthase: (7R,8S)-diaminononanoate + ATP + CO2→dethiobiotin + ADP + 3 H+ + phosphate (EC 6.3.3.3)
b0778 (bioD)	-0.8
b1593 (b1593)	-0.4
Biotin synthase: dethiobiotin + H+ + 2 a reduced [2Fe-2S] ferredoxin + S-adenosyl-L-methionine + a sulfurated [sulfur carrier]→4,5-secobiotin + 5'-deoxyadenosine + L-methionine + 2 an oxidized [2Fe-2S] ferredoxin + an unsulfurated [sulfur carrier] (EC 2.8.1.6)
b0775 (bioB)	-1.8
Biotin synthase: 4,5-secobiotin + S-adenosyl-L-methionine→biotin + 5'-deoxyadenosine + L-methionine + H+ (EC 2.8.1.6)
b0775 (bioB)	-1.8

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information