MetaCyc Pathway: superpathway of L-tryptophan biosynthesis in Pseudomonas sp. RS175

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Reactions and Genes Shikimic Acid carbon 10 mM
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3-deoxy-7-phosphoheptulonate synthase:
D-erythrose 4-phosphate + phosphoenolpyruvate + H2O→3-deoxy-D-arabino-heptulosonate 7-phosphate + phosphate (EC 2.5.1.54)
PFR28_00962 +0.1
PFR28_00978 +0.0
PFR28_01407 -0.0
3-dehydroquinate synthase:
3-deoxy-D-arabino-heptulosonate 7-phosphate→3-dehydroquinate + phosphate (EC 4.2.3.4)
PFR28_01544 +0.1
PFR28_05013 N.D.
3-dehydroquinate dehydratase:
3-dehydroquinate→3-dehydroshikimate + H2O (EC 4.2.1.10)
PFR28_00416 N.D.
PFR28_03810 -4.8
PFR28_05222 N.D.
Shikimate dehydrogenase (in reverse):
3-dehydroshikimate + NADPH + H+→shikimate + NADP+ (EC 1.1.1.25)
PFR28_01211 +0.2
PFR28_03811 -3.4
PFR28_04655 N.D.
Shikimate kinase:
shikimate + ATP→shikimate 3-phosphate + ADP + H+ (EC 2.7.1.71)
PFR28_05012 N.D.
3-phosphoshikimate 1-carboxyvinyltransferase:
shikimate 3-phosphate + phosphoenolpyruvate→5-enolpyruvoyl-shikimate 3-phosphate + phosphate (EC 2.5.1.19)
PFR28_00909 -0.1
Chorismate synthase:
5-enolpyruvoyl-shikimate 3-phosphate→chorismate + phosphate (EC 4.2.3.5)
PFR28_03126 N.D.
Anthranilate synthase:
chorismate + L-glutamine→anthranilate + L-glutamate + H+ + pyruvate (EC 4.1.3.27)
PFR28_01422 +0.1
PFR28_01423 +0.1
PFR28_04045 N.D.
PFR28_04047 N.D.
Anthranilate phosphoribosyltransferase (in reverse):
anthranilate + 5-phospho-α-D-ribose 1-diphosphate→N-(5-phosphoribosyl)-anthranilate + diphosphate (EC 2.4.2.18)
PFR28_02601 +0.1
PFR28_04044 N.D.
Phosphoribosylanthranilate isomerase:
N-(5-phosphoribosyl)-anthranilate→1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate (EC 5.3.1.24)
PFR28_02816 N.D.
Indole-3-glycerol-phosphate synthase:
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H+→(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O (EC 4.1.1.48)
PFR28_04043 N.D.
Indole-3-glycerol-phosphate lyase:
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate→indole + D-glyceraldehyde 3-phosphate (EC 4.1.2.8; 4.2.1.20)
PFR28_03639 +0.1
PFR28_04715 N.D.
PFR28_04716 N.D.
PFR28_04784 N.D.
indole + L-serine→L-tryptophan + H2O (EC 4.2.1.122; 4.2.1.20)
PFR28_03639 +0.1
PFR28_04715 N.D.
PFR28_04716 N.D.
PFR28_04784 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information