MetaCyc Pathway: oleate biosynthesis IV (anaerobic) in Variovorax sp. OAS795

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Reactions and Genes D-Arabinose (C)
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3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydodecanoyl-[acp]→a (2E)-dodec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
ABID97_RS17115 N.D.
Trans-2-decenoyl-[acyl-carrier-protein] isomerase:
a (2E)-dodec-2-enoyl-[acp]→a (3Z)-dodec-3-enoyl-[acp] (EC 5.3.3.14)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (3Z)-dodec-3-enoyl-[acp] + a malonyl-[acp] + H+→a (5Z)-3-oxo-tetradec-5-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
ABID97_RS02835 -0.1
ABID97_RS03825 +0.1
ABID97_RS08320 N.D.
ABID97_RS11000 -0.1
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (5Z)-3-oxo-tetradec-5-enoyl-[acp][cytosol] + NADPH[cytosol] + H+[cytosol]→a (3R,5Z)-3-hydroxy-tetradec-5-enoyl-[acp][cytosol] + NADP+[cytosol] (EC 1.1.1.100)
ABID97_RS01400 -0.0
ABID97_RS03055 +0.1
ABID97_RS03260 N.D.
ABID97_RS04295 -0.5
ABID97_RS05575 +0.1
ABID97_RS05830 +2.0
ABID97_RS08310 N.D.
ABID97_RS08870 N.D.
ABID97_RS10320 -0.3
ABID97_RS11250 +0.1
ABID97_RS11585 N.D.
ABID97_RS11630 -0.1
ABID97_RS14425 +0.0
ABID97_RS14760 +0.6
ABID97_RS15765 +0.2
ABID97_RS16045 N.D.
ABID97_RS16765 +0.4
ABID97_RS17320 +0.0
ABID97_RS18630 -0.1
ABID97_RS18660 -0.0
ABID97_RS18980 +0.1
ABID97_RS18985 -0.3
ABID97_RS18990 +0.6
ABID97_RS22670 N.D.
ABID97_RS24025 -0.2
ABID97_RS25995 N.D.
ABID97_RS26005 N.D.
ABID97_RS26430 N.D.
ABID97_RS27295 N.D.
ABID97_RS28245 -0.8
ABID97_RS28425 -0.1
ABID97_RS28765 N.D.
ABID97_RS29505 -0.5
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,5Z)-3-hydroxy-tetradec-5-enoyl-[acp][cytosol]→a (2E,5Z)-tetradeca-2,5-dienoyl-[acp][cytosol] + H2O[cytosol] (EC 4.2.1.59)
ABID97_RS17115 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,5Z)-tetradeca-2,5-dienoyl-[acp][cytosol] + NADH[cytosol] + H+[cytosol]→a (5Z)-tetradec-5-enoyl-[acp][cytosol] + NAD+[cytosol] (EC 1.3.1.9)
ABID97_RS16525 N.D.
ABID97_RS19675 -0.2
ABID97_RS27875 N.D.
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (5Z)-tetradec-5-enoyl-[acp] + a malonyl-[acp] + H+→a (7Z)-3-oxo-hexadec-7-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
ABID97_RS02835 -0.1
ABID97_RS03825 +0.1
ABID97_RS08320 N.D.
ABID97_RS11000 -0.1
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (7Z)-3-oxo-hexadec-7-enoyl-[acp][cytosol] + NADPH[cytosol] + H+[cytosol]→a (3R,7Z)-3-hydroxy-hexadec-7-enoyl-[acp][cytosol] + NADP+[cytosol] (EC 1.1.1.100)
ABID97_RS01400 -0.0
ABID97_RS03055 +0.1
ABID97_RS03260 N.D.
ABID97_RS04295 -0.5
ABID97_RS05575 +0.1
ABID97_RS05830 +2.0
ABID97_RS08310 N.D.
ABID97_RS08870 N.D.
ABID97_RS10320 -0.3
ABID97_RS11250 +0.1
ABID97_RS11585 N.D.
ABID97_RS11630 -0.1
ABID97_RS14425 +0.0
ABID97_RS14760 +0.6
ABID97_RS15765 +0.2
ABID97_RS16045 N.D.
ABID97_RS16765 +0.4
ABID97_RS17320 +0.0
ABID97_RS18630 -0.1
ABID97_RS18660 -0.0
ABID97_RS18980 +0.1
ABID97_RS18985 -0.3
ABID97_RS18990 +0.6
ABID97_RS22670 N.D.
ABID97_RS24025 -0.2
ABID97_RS25995 N.D.
ABID97_RS26005 N.D.
ABID97_RS26430 N.D.
ABID97_RS27295 N.D.
ABID97_RS28245 -0.8
ABID97_RS28425 -0.1
ABID97_RS28765 N.D.
ABID97_RS29505 -0.5
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,7Z)-3-hydroxy-hexadec-7-enoyl-[acp][cytosol]→a (2E,7Z)-hexadeca-2,7-dienoyl-[acp][cytosol] + H2O[cytosol] (EC 4.2.1.59)
ABID97_RS17115 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,7Z)-hexadeca-2,7-dienoyl-[acp][cytosol] + NADH[cytosol] + H+[cytosol]→a (7Z)-hexadec-7-enoyl-[acp][cytosol] + NAD+[cytosol] (EC 1.3.1.9)
ABID97_RS16525 N.D.
ABID97_RS19675 -0.2
ABID97_RS27875 N.D.
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (7Z)-hexadec-7-enoyl-[acp] + a malonyl-[acp] + H+→a (9Z)-3-oxo-octadec-9-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
ABID97_RS02835 -0.1
ABID97_RS03825 +0.1
ABID97_RS08320 N.D.
ABID97_RS11000 -0.1
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (9Z)-3-oxo-octadec-9-enoyl-[acp][cytosol] + NADPH[cytosol] + H+[cytosol]→a (3R,9Z)-3-hydroxy-octadec-9-enoyl-[acp][cytosol] + NADP+[cytosol] (EC 1.1.1.100)
ABID97_RS01400 -0.0
ABID97_RS03055 +0.1
ABID97_RS03260 N.D.
ABID97_RS04295 -0.5
ABID97_RS05575 +0.1
ABID97_RS05830 +2.0
ABID97_RS08310 N.D.
ABID97_RS08870 N.D.
ABID97_RS10320 -0.3
ABID97_RS11250 +0.1
ABID97_RS11585 N.D.
ABID97_RS11630 -0.1
ABID97_RS14425 +0.0
ABID97_RS14760 +0.6
ABID97_RS15765 +0.2
ABID97_RS16045 N.D.
ABID97_RS16765 +0.4
ABID97_RS17320 +0.0
ABID97_RS18630 -0.1
ABID97_RS18660 -0.0
ABID97_RS18980 +0.1
ABID97_RS18985 -0.3
ABID97_RS18990 +0.6
ABID97_RS22670 N.D.
ABID97_RS24025 -0.2
ABID97_RS25995 N.D.
ABID97_RS26005 N.D.
ABID97_RS26430 N.D.
ABID97_RS27295 N.D.
ABID97_RS28245 -0.8
ABID97_RS28425 -0.1
ABID97_RS28765 N.D.
ABID97_RS29505 -0.5
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,9Z)-3-hydroxy-octadec-9-enoyl-[acp][cytosol]→a (2E,9Z)-octadeca-2,9-dienoyl-[acp][cytosol] + H2O[cytosol] (EC 4.2.1.59)
ABID97_RS17115 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,9Z)-octadeca-2,9-dienoyl-[acp] + NADH + H+→an oleoyl-[acp] + NAD+ (EC 1.3.1.9)
ABID97_RS16525 N.D.
ABID97_RS19675 -0.2
ABID97_RS27875 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information