MetaCyc Pathway: L-isoleucine degradation I in Pseudomonas simiae WCS417

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Reactions and Genes Parafilmed volatile agar plate with no fungus
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Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)
PS417_17565 -0.4
2-oxoisovalerate dehydrogenase (acylating):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + NAD+→(2S)-2-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
PS417_13840 +0.0
PS417_13850 -0.4
(E)-2-methylcrotonoyl-CoA + H2O→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (EC 4.2.1.150)
PS417_07030 -0.1
PS417_07575 +0.0
PS417_08535 -0.0
PS417_10680 +0.4
PS417_11445 +3.9
PS417_21215 -0.1
PS417_22625 -0.1
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+→2-methylacetoacetyl-CoA + NADH + H+ (EC 1.1.1.178)
PS417_07575 +0.0
PS417_13860 N.D.
PS417_21215 -0.1
Acetyl-CoA C-acyltransferase (in reverse):
2-methylacetoacetyl-CoA + coenzyme A→acetyl-CoA + propanoyl-CoA (EC 2.3.1.16)
PS417_06710 -0.5
PS417_07580 N.D.
PS417_10515 +0.1
PS417_13855 -0.3
PS417_15175 N.D.
PS417_19210 -0.0
PS417_21220 -0.1
PS417_03145 -0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information