MetaCyc Pathway: L-glutamate degradation VII (to butanoate) in Pseudomonas simiae WCS417

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Reactions and Genes Parafilmed volatile agar plate with no fungus
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Methylaspartate mutase (in reverse):
L-glutamate→(2S, 3S)-3-methylaspartate (EC 5.4.99.1)
No genes
Methylaspartate ammonia-lyase:
(2S, 3S)-3-methylaspartate→mesaconate + ammonium (EC 4.3.1.2)
No genes
(S)-2-methylmalate dehydratase (in reverse):
mesaconate + H2O→(S)-citramalate (EC 4.2.1.34)
No genes
Citramalate lyase:
(S)-citramalate→pyruvate + acetate (EC 4.1.3.22)
No genes
Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + 2 a reduced ferredoxin [iron-sulfur] cluster + CO2 + H+ (EC 1.2.7.1)
No genes
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
PS417_06710 -0.5
PS417_07580 N.D.
PS417_10515 +0.1
PS417_13855 -0.3
PS417_15175 N.D.
PS417_19210 -0.0
PS417_21220 -0.1
PS417_03145 -0.2
Ferredoxin hydrogenase (in reverse):
2 a reduced ferredoxin [iron-sulfur] cluster + 2 H+→H2 + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.12.7.2)
No genes
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
PS417_07575 +0.0
PS417_21215 -0.1
PS417_26285 -0.1
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
PS417_07030 -0.1
PS417_07575 +0.0
PS417_08535 -0.0
PS417_10680 +0.4
PS417_11445 +3.9
PS417_22625 -0.1
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] + butanoyl-CoA[cytosol] + 2 NAD+[cytosol] (EC 1.3.1.109)
No genes
Phosphate butyryltransferase:
butanoyl-CoA + phosphate→butanoyl phosphate + coenzyme A (EC 2.3.1.19)
No genes
Branched-chain-fatty-acid kinase (in reverse):
butanoyl phosphate + ADP→butanoate + ATP (EC 2.7.2.14; 2.7.2.7)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information