MetaCyc Pathway: superpathway of L-lysine, L-threonine and L-methionine biosynthesis I in Pseudomonas simiae WCS417

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Reactions and Genes Carbon source L-Ornithine 5 mM
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate
(EC 2.6.1.1)
PS417_08560 +0.2
PS417_09070 -0.0
PS417_09120 +0.0
PS417_11840 -0.2
PS417_12775 -0.0
PS417_12985 +0.0
PS417_13545 -0.3
PS417_14865 -0.1
PS417_15040 -0.0
PS417_15990 -0.4
PS417_19025 +0.1
PS417_19385 -0.3
PS417_19910 -0.1
PS417_21030 +0.2
PS417_21275 +0.0
PS417_23120 +0.0
PS417_27730 +0.1
PS417_28165 +0.0
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP
(EC 2.7.2.4)
PS417_21655 -0.8
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate
(EC 1.2.1.11)
PS417_06650 N.D.
PS417_18575 -0.0
PS417_18580 N.D.
L-aspartate 4-semialdehyde + pyruvate→(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H+ + H2O (EC 4.3.3.7)
PS417_06885 -0.1
PS417_07165 -1.1
PS417_14640 N.D.
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+
(EC 1.1.1.3)
PS417_22885 N.D.
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+
(EC 2.7.1.39)
PS417_21120 -0.2
PS417_00395 N.D.
Homoserine O-succinyltransferase:
L-homoserine + succinyl-CoA→O-succinyl-L-homoserine + coenzyme A
(EC 2.3.1.46)
No genes
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+→(S)-2,3,4,5-tetrahydrodipicolinate + NAD(P)+ + H2O (EC 1.17.1.8)
PS417_24050 N.D.
Cystathionine gamma-synthase:
O-succinyl-L-homoserine + L-cysteine→L-cystathionine + H+ + succinate
(EC 2.5.1.48)
PS417_18530 -2.6
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase:
(S)-2,3,4,5-tetrahydrodipicolinate + succinyl-CoA + H2ON-succinyl-2-amino-6-ketopimelate + coenzyme A
(EC 2.3.1.117)
PS417_06180 N.D.
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate
(EC 4.2.3.1)
PS417_22880 N.D.
Cysteine-S-conjugate beta-lyase:
L-cystathionine + H2O→L-homocysteine + ammonium + pyruvate
(EC 4.4.1.13)
No genes
Succinyldiaminopimelate transaminase (in reverse):
N-succinyl-2-amino-6-ketopimelate + L-glutamateN-succinyl-L,L-2,6-diaminopimelate + 2-oxoglutarate
(EC 2.6.1.17)
PS417_06195 +0.2
PS417_21710 -1.9
PS417_25950 +0.1
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase:
L-homocysteine + 5-methyltetrahydropteroyl tri-L-glutamate→L-methionine + tetrahydropteroyl tri-L-glutamate
(EC 2.1.1.14)
PS417_18775 +0.3
Methionine synthase:
L-homocysteine + a 5-methyltetrahydrofolate→L-methionine + a tetrahydrofolate
(EC 2.1.1.13)
PS417_12310 -1.3
Succinyl-diaminopimelate desuccinylase:
N-succinyl-L,L-2,6-diaminopimelate + H2O→L,L-diaminopimelate + succinate
(EC 3.5.1.18)
PS417_06155 N.D.
Diaminopimelate epimerase:
L,L-diaminopimelate→meso-diaminopimelate
(EC 5.1.1.7)
PS417_09775 +0.3
PS417_12450 +0.1
PS417_15365 +0.3
PS417_22110 N.D.
PS417_27585 -0.3
Diaminopimelate decarboxylase:
meso-diaminopimelate + H+→L-lysine + CO2
(EC 4.1.1.20)
PS417_27580 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information