MetaCyc Pathway: 3-phenylpropanoate degradation in Pseudomonas putida KT2440

Reactions and Genes	Butanol (C)
3-phenylpropanoate + ATP + coenzyme A→3-phenylpropanoyl-CoA + AMP + diphosphate
No genes
3-phenylpropanoyl-CoA + an oxidized electron carrier→(E)-cinnamoyl-CoA + a reduced two electron carrier
No genes
Enoyl-CoA hydratase (in reverse): (E)-cinnamoyl-CoA + H2O→(S)-3-hydroxy-3-phenylpropanoyl-CoA (EC 4.2.1.17)
PP_1412	+0.0
PP_1845	-0.1
PP_2047	+0.0
PP_2136	-1.5
PP_2217	-1.3
PP_3283	+0.2
PP_3284	+0.0
PP_3358	+0.0
PP_3491	-0.1
PP_3726	-0.5
PP_3732	+0.3
PP_4030	+0.1
3-hydroxyacyl-CoA dehydrogenase: (S)-3-hydroxy-3-phenylpropanoyl-CoA + NAD+→3-oxo-3-phenylpropanoyl-CoA + NADH + H+ (EC 1.1.1.35)
PP_2047	+0.0
PP_2136	-1.5
Acetyl-CoA C-acyltransferase (in reverse): 3-oxo-3-phenylpropanoyl-CoA + coenzyme A→benzoyl-CoA + acetyl-CoA (EC 2.3.1.16)
PP_0582	+0.0
PP_1377	+0.1
PP_2051	-0.0
PP_2137	-1.1
PP_2215	+0.4
PP_3280	+0.1
PP_3355	+0.1
PP_3754	-2.2
PP_4636	+0.1
Benzoyl-CoA 3-monooxygenase: benzoyl-CoA + NADPH + dioxygen + H+→3-hydroxybenzoyl-CoA + NADP+ + H2O (EC 1.14.13.58)
No genes
3-hydroxybenzoyl-CoA + a reduced two electron carrier + dioxygen→gentisate + an oxidized electron carrier + coenzyme A + H+
No genes
Gentisate 1,2-dioxygenase: gentisate + dioxygen→3-maleylpyruvate + H+ (EC 1.13.11.4)
No genes
Maleylpyruvate isomerase: 3-maleylpyruvate→3-fumarylpyruvate (EC 5.2.1.4)
PP_4619	+0.0
3-fumarylpyruvate + H2O→fumarate + pyruvate + H+ (EC 3.7.1.20)
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information