MetaCyc Pathway: L-isoleucine biosynthesis I (from threonine) in Pectobacterium carotovorum WPP14

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Reactions and Genes M9+glucose
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Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
HER17_RS01080 -3.5
HER17_RS09820 -0.5
HER17_RS13855 +0.0
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
HER17_RS01080 -3.5
HER17_RS07405 +0.2
HER17_RS09820 -0.5
HER17_RS13855 +0.0
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
HER17_RS01080 -3.5
HER17_RS07405 +0.2
HER17_RS09820 -0.5
HER17_RS13855 +0.0
HER17_RS19520 -1.0
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
HER17_RS00335 -0.1
HER17_RS01060 -0.9
HER17_RS01065 -1.3
HER17_RS03010 +0.1
HER17_RS03015 -0.2
HER17_RS03095 -0.7
HER17_RS03100 -1.3
HER17_RS18035 +0.3
HER17_RS19205 +0.2
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
HER17_RS01100 -5.6
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
HER17_RS01075 -4.9
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
HER17_RS01070 -1.3
HER17_RS07450 +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information