MetaCyc Pathway: L-leucine degradation I in Pseudomonas putida KT2440

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Reactions and Genes 4-Methyl-2-oxopentanoic (C)
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Branched-chain-amino-acid transaminase:
L-leucine + 2-oxoglutarate→4-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42; 2.6.1.6)
PP_3511 -6.6
2-oxoisovalerate dehydrogenase (acylating):
4-methyl-2-oxopentanoate + coenzyme A + NAD+→3-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
3-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→3-methylcrotonyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.4)
PP_2437 +0.1
PP_4064 -4.6
Methylcrotonoyl-CoA carboxylase:
3-methylcrotonyl-CoA + ATP + hydrogen carbonate→3-methylglutaconyl-CoA + ADP + H+ + phosphate (EC 6.4.1.4)
PP_4065 -5.0
PP_4067 -5.2
Methylglutaconyl-CoA hydratase (in reverse):
3-methylglutaconyl-CoA + H2O→(S)-3-hydroxy-3-methylglutaryl-CoA (EC 4.2.1.18)
PP_4066 -5.7
Hydroxymethylglutaryl-CoA lyase:
(S)-3-hydroxy-3-methylglutaryl-CoA→acetoacetate + acetyl-CoA (EC 4.1.3.4)
PP_3393 -0.1
PP_3394 +0.3
PP_3540 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information