MetaCyc Pathway: superpathway of heme b biosynthesis from glutamate in Escherichia coli BW25113

Reactions and Genes	2-nitrobenzenesulfonyl fluoride
Glutamate--tRNA ligase: L-glutamate + ATP + a tRNAglu→an L-glutamyl-[tRNAGlu] + AMP + diphosphate (EC 6.1.1.17)
b2400 (gltX)	N.D.
Glutamyl-tRNA reductase (in reverse): an L-glutamyl-[tRNAGlu] + NADPH + H+→(S)-4-amino-5-oxopentanoate + a tRNAglu + NADP+ (EC 1.2.1.70)
b1210 (hemA)	N.D.
Glutamate-1-semialdehyde 2,1-aminomutase: (S)-4-amino-5-oxopentanoate→5-aminolevulinate (EC 5.4.3.8)
b0154 (hemL)	N.D.
Porphobilinogen synthase: 2 5-aminolevulinate→porphobilinogen + H+ + 2 H2O (EC 4.2.1.24)
b0369 (hemB)	N.D.
Hydroxymethylbilane synthase: 4 porphobilinogen + H2O→preuroporphyrinogen + 4 ammonium (EC 2.5.1.61)
b3805 (hemC)	N.D.
Uroporphyrinogen-III synthase: preuroporphyrinogen→uroporphyrinogen-III + H2O (EC 4.2.1.75)
b3804 (hemD)	N.D.
Uroporphyrinogen decarboxylase: uroporphyrinogen-III + 4 H+→coproporphyrinogen III + 4 CO2 (EC 4.1.1.37)
b3997 (hemE)	N.D.
Coproporphyrinogen oxidase: coproporphyrinogen III + dioxygen + 2 H+→protoporphyrinogen IX + 2 CO2 + 2 H2O (EC 1.3.3.3)
b2436 (hemF)	-0.0
Protoporphyrinogen oxidase: protoporphyrinogen IX + 3 dioxygen→protoporphyrin IX + 3 hydrogen peroxide (EC 1.3.3.4)
b3850 (hemG)	N.D.
protoporphyrin IX + Fe2+→protoheme + 2 H+ (EC 4.98.1.1)
b0475 (hemH)	N.D.
b1019 (ycdB)	-0.0
b2431 (b2431)	-2.4

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information