MetaCyc Pathway: pyrimidine deoxyribonucleotides biosynthesis from CTP in Escherichia coli BW25113
| Reactions and Genes | Nalidixic 0.06 mM |
|---|---|
| remove | |
| Nucleoside-triphosphatase: CTP + H2O→CDP + H+ + phosphate (EC 3.6.1.15; 3.6.1.5) | |
| b2954 (yggV) | -1.2 |
| Ribonucleoside-diphosphate reductase (in reverse): CDP + a reduced thioredoxin→dCDP + an oxidized thioredoxin + H2O (EC 1.17.4.1) | |
| b2234 (nrdA) | N.D. |
| b2235 (nrdB) | N.D. |
| b2674 (nrdI) | +0.1 |
| b2675 (nrdE) | +0.1 |
| b2676 (nrdF) | -0.1 |
| Nucleoside-diphosphate kinase: dCDP + ATP→dCTP + ADP (EC 2.7.4.6) | |
| b0474 (adk) | N.D. |
| b2518 (ndk) | +1.5 |
| Apyrase: dCDP + H2O→dCMP + H+ + phosphate (EC 3.6.1.5; 3.6.1.6) | |
| No genes | |
| dCMP deaminase: dCMP + H+ + H2O→dUMP + ammonium (EC 3.5.4.12) | |
| No genes | |
| Thymidylate synthase: dUMP + a 5,10-methylenetetrahydrofolate→dTMP + a 7,8-dihydrofolate (EC 2.1.1.45) | |
| b2827 (thyA) | N.D. |
| T(2)-induced deoxynucleotide kinase: dTMP + ATP→dTDP + ADP (EC 2.7.4.12; 2.7.4.13; 2.7.4.9) | |
| b1098 (tmk) | N.D. |
| Nucleoside-diphosphate kinase: dTDP + ATP→dTTP + ADP (EC 2.7.4.6) | |
| b0474 (adk) | N.D. |
| b2518 (ndk) | +1.5 |
Links:
- Pathway details at MetaCyc
- Fitness data for 10 genes
- All MetaCyc pathways for Escherichia coli BW25113
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information