MetaCyc Pathway: methyl ketone biosynthesis (engineered) in Pseudomonas putida KT2440

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Reactions and Genes 3-Hydroxypropionate (C)
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a fatty acid + ATP + coenzyme A→an acyl-CoA + AMP + diphosphate
No genes
Acyl-CoA oxidase:
a 2,3,4-saturated fatty acyl CoA + dioxygen→a (2E)-2-enoyl-CoA + hydrogen peroxide
(EC 1.3.3.6)
No genes
Enoyl-CoA hydratase (in reverse):
a (2E)-2-enoyl-CoA + H2O→a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA
(EC 4.2.1.17)
PP_1412 -0.1
PP_1845 +0.3
PP_2047 +0.2
PP_2136 -0.5
PP_2217 -1.2
PP_3283 +0.2
PP_3284 -0.1
PP_3358 +0.1
PP_3491 -2.0
PP_3726 -0.4
PP_3732 +0.1
PP_4030 -0.2
3-hydroxyacyl-CoA dehydrogenase:
a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA + NAD+→a 2,3,4-saturated 3-oxoacyl-CoA + NADH + H+
(EC 1.1.1.35)
PP_2047 +0.2
PP_2136 -0.5
a 2,3,4-saturated 3-oxoacyl-CoA + H2O→a 3-oxoacid + coenzyme A + H+
No genes
a 3-oxoacid + H+→a methyl ketone + CO2 (spontaneous)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information