MetaCyc Pathway: 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) in Pseudomonas putida KT2440

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Reactions and Genes benzoic (C)
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9-cis, 11-trans-octadecadienoyl-CoA + 2 coenzyme A + 2 NAD+ + 2 dioxygen + 2 H2O→5-cis, 7-trans-tetradecadienoyl-CoA + 2 acetyl-CoA + 2 hydrogen peroxide + 2 NADH + 2 H+
No genes
Acyl-CoA oxidase:
5-cis, 7-trans-tetradecadienoyl-CoA + dioxygen→2-trans, 5-cis, 7-trans-tetradecatrienoyl-CoA + hydrogen peroxide
(EC 1.3.3.6)
No genes
2-trans, 5-cis, 7-trans-tetradecatrienoyl-CoA + H2O→(3R)-hydroxy- 5-cis, 7-trans-tetradecadienoyl-CoA (EC 4.2.1.119)
No genes
(3R)-hydroxy- 5-cis, 7-trans-tetradecadienoyl-CoA + NAD+→5-cis, 7-trans-3-oxo-tetradecadienoyl-CoA + NADH + H+ (EC 1.1.1.M19)
No genes
Acetyl-CoA C-acyltransferase (in reverse):
5-cis, 7-trans-3-oxo-tetradecadienoyl-CoA + coenzyme A→(3Z,5E)-dodec-3,5-dienoyl-CoA + acetyl-CoA
(EC 2.3.1.16)
PP_0582 +0.0
PP_1377 -6.6
PP_2051 +0.1
PP_2137 -0.5
PP_2215 +0.2
PP_3280 -0.3
PP_3355 -0.1
PP_3754 +0.3
PP_4636 -0.1
Dodecenoyl-CoA isomerase:
(3Z,5E)-dodec-3,5-dienoyl-CoA→(2E,5E)-dodeca-2,5-dienoyl-CoA
(EC 5.3.3.8)
PP_2047 -0.0
PP_2136 -1.6
(2E,5E)-dodeca-2,5-dienoyl-CoA + H2O→(3R)-hydroxy-5-trans-dodecenoyl-CoA (EC 4.2.1.119)
No genes
(3R)-hydroxy-5-trans-dodecenoyl-CoA + NAD+→(5E)-3-oxo-dodec-5-enoyl-CoA + NADH + H+ (EC 1.1.1.M19)
No genes
Acetyl-CoA C-acyltransferase (in reverse):
(5E)-3-oxo-dodec-5-enoyl-CoA + coenzyme A→(3E)-dec-3-enoyl-CoA + acetyl-CoA
(EC 2.3.1.16)
PP_0582 +0.0
PP_1377 -6.6
PP_2051 +0.1
PP_2137 -0.5
PP_2215 +0.2
PP_3280 -0.3
PP_3355 -0.1
PP_3754 +0.3
PP_4636 -0.1
Dodecenoyl-CoA isomerase:
(3E)-dec-3-enoyl-CoA→(2E)-dec-2-enoyl-CoA
(EC 5.3.3.8)
PP_2047 -0.0
PP_2136 -1.6

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information