MetaCyc Pathway: purine nucleotides degradation II (aerobic) in Escherichia coli BW25113

Reactions and Genes	Trimethoprim 0.0025 mM
5'-nucleotidase: AMP + H2O→adenosine + phosphate (EC 3.1.3.5)
b0383 (phoA)	-0.0
b0480 (ushA)	-0.2
b2291 (yfbR)	+0.2
b2744 (surE)	-1.3
b4055 (aphA)	-0.9
b4374 (yjjG)	-2.4
IMP dehydrogenase: IMP + NAD+ + H2O→XMP + NADH + H+ (EC 1.1.1.205)
b2508 (guaB)	N.D.
5'-nucleotidase: GMP + H2O→guanosine + phosphate (EC 3.1.3.5)
b0480 (ushA)	-0.2
b2291 (yfbR)	+0.2
b2744 (surE)	-1.3
b3399 (yrfG)	+0.4
b4055 (aphA)	-0.9
b4374 (yjjG)	-2.4
Adenosine deaminase: adenosine + H+ + H2O→inosine + ammonium (EC 3.5.4.4)
b1623 (add)	-1.2
b2593 (yfiH)	-1.3
Purine-nucleoside phosphorylase: guanosine + phosphate→guanine + α-D-ribose-1-phosphate (EC 2.4.2.1; 2.4.2.15)
b0391 (yaiE)	+0.0
b2407 (xapA)	+0.3
b4384 (deoD)	-0.6
5'-nucleotidase: XMP + H2O→xanthosine + phosphate (EC 3.1.3.5)
b0480 (ushA)	-0.2
b2291 (yfbR)	+0.2
b2744 (surE)	-1.3
b4055 (aphA)	-0.9
b4374 (yjjG)	-2.4
Guanine deaminase: guanine + H+ + H2O→xanthine + ammonium (EC 3.5.4.3)
b2883 (guaD)	+0.3
Purine-nucleoside phosphorylase: inosine + phosphate→hypoxanthine + α-D-ribose-1-phosphate (EC 2.4.2.1)
b0391 (yaiE)	+0.0
b2407 (xapA)	+0.3
b2593 (yfiH)	-1.3
b4384 (deoD)	-0.6
Purine-nucleoside phosphorylase: xanthosine + phosphate→xanthine + α-D-ribose-1-phosphate (EC 2.4.2.1)
b0391 (yaiE)	+0.0
b2407 (xapA)	+0.3
b4384 (deoD)	-0.6
Xanthine dehydrogenase: hypoxanthine + NAD+ + H2O→xanthine + NADH + H+ (EC 1.17.1.4)
b0284 (yagR)	-0.0
b0285 (yagS)	+0.2
b2866 (xdhA)	-0.1
b2867 (xdhB)	+0.2
b2868 (xdhC)	+0.1
Xanthine dehydrogenase: xanthine + NAD+ + H2O→urate + NADH + H+ (EC 1.17.1.4)
b0284 (yagR)	-0.0
b0285 (yagS)	+0.2
b2866 (xdhA)	-0.1
b2867 (xdhB)	+0.2
b2868 (xdhC)	+0.1

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information