MetaCyc Pathway: TCA cycle I (prokaryotic) in Escherichia coli BL21

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Reactions and Genes Casamino-acids
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2-oxoglutarate + coenzyme A + NAD+→succinyl-CoA + CO2 + NADH (EC 1.2.1.105)
ECD_00115 N.D.
Succinate--CoA ligase (ADP-forming) (in reverse):
succinyl-CoA + ADP + phosphate→succinate + ATP + coenzyme A (EC 6.2.1.5)
ECD_00687 -3.3
ECD_00688 -3.0
Succinate dehydrogenase (ubiquinone):
succinate[in] + an electron-transfer quinone→fumarate[in] + an electron-transfer quinol (EC 1.3.5.1)
No genes
Fumarate hydratase (in reverse):
fumarate + H2O→(S)-malate (EC 4.2.1.2)
ECD_01580 N.D.
ECD_01581 -0.5
ECD_01644 N.D.
ECD_02760 -0.2
ECD_03993 +0.1
Malate dehydrogenase:
(S)-malate + NAD+→oxaloacetate + NADH + H+ (EC 1.1.1.37; 1.1.1.38)
ECD_00768 -0.2
ECD_01437 N.D.
ECD_03096 -3.4
(S)-malate[in] + an electron-transfer quinone→oxaloacetate[in] + an electron-transfer quinol (EC 1.1.5.4)
ECD_02137 +0.2
Citrate (Si)-synthase:
oxaloacetate + acetyl-CoA + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
ECD_00680 -1.1
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
ECD_00117 N.D.
ECD_01253 +0.2
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
ECD_00117 N.D.
ECD_01253 +0.2
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→2-oxoglutarate + CO2 + NADPH (EC 1.1.1.42)
ECD_01134 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information