MetaCyc Pathway: 5,6-dehydrokavain biosynthesis (engineered) in Escherichia coli BW25113

Reactions and Genes	LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
3-phenylpropanoate + ATP + coenzyme A→3-phenylpropanoyl-CoA + AMP + diphosphate
b1805 (fadD)	+1.4
trans-cinnamate + ATP + coenzyme A→(E)-cinnamoyl-CoA + AMP + diphosphate
b1805 (fadD)	+1.4
Acetyl-CoA C-acyltransferase: (E)-cinnamoyl-CoA + acetyl-CoA→(4E)-3-oxo-5-phenylpent-4-enoyl-CoA + coenzyme A (EC 2.3.1.16)
b1397 (paaJ)	+0.4
b2224 (atoB)	-0.0
b2342 (yfcY)	-0.0
b2844 (yqeF)	+0.1
b3845 (fadA)	-0.2
Acetyl-CoA C-acyltransferase: 3-phenylpropanoyl-CoA + acetyl-CoA→3-oxo-5-phenylpentanoyl-CoA + coenzyme A (EC 2.3.1.16)
b1397 (paaJ)	+0.4
b2224 (atoB)	-0.0
b2342 (yfcY)	-0.0
b2844 (yqeF)	+0.1
b3845 (fadA)	-0.2
Acetyl-CoA C-acyltransferase: (4E)-3-oxo-5-phenylpent-4-enoyl-CoA + acetyl-CoA→(6E)-3,5-dioxo-7-phenylhept-6-enoyl-CoA + coenzyme A (EC 2.3.1.16)
b1397 (paaJ)	+0.4
b2224 (atoB)	-0.0
b2342 (yfcY)	-0.0
b2844 (yqeF)	+0.1
b3845 (fadA)	-0.2
Acetyl-CoA C-acyltransferase: 3-oxo-5-phenylpentanoyl-CoA + acetyl-CoA→3,5-dioxo-7-phenylheptanoyl-CoA + coenzyme A (EC 2.3.1.16)
b1397 (paaJ)	+0.4
b2224 (atoB)	-0.0
b2342 (yfcY)	-0.0
b2844 (yqeF)	+0.1
b3845 (fadA)	-0.2
(6E)-3,5-dioxo-7-phenylhept-6-enoyl-CoA→(E)-4-hydroxy-6-(styryl)pyran-2-one + coenzyme A (spontaneous)
No genes
3,5-dioxo-7-phenylheptanoyl-CoA→4-hydroxy-6-(penethyl)pyran-2-one + coenzyme A (spontaneous)
No genes
(E)-4-hydroxy-6-(styryl)pyran-2-one + S-adenosyl-L-methionine→5,6-dehydrokavain + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.M66)
No genes
4-hydroxy-6-(penethyl)pyran-2-one + S-adenosyl-L-methionine→dihydro-5,6-dehydrokavain + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.M66)
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information