MetaCyc Pathway: dTDP-sibirosamine biosynthesis in Escherichia coli BW25113

Reactions and Genes	LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
Glucose-1-phosphate thymidylyltransferase: α-D-glucopyranose 1-phosphate + H+ + dTTP→dTDP-α-D-glucose + diphosphate (EC 2.7.7.24)
b0363 (yaiP)	-0.0
b2039 (rfbA)	+0.4
b3789 (rffH)	+0.7
dTDP-glucose 4,6-dehydratase: dTDP-α-D-glucose→dTDP-4-dehydro-6-deoxy-α-D-glucopyranose + H2O (EC 4.2.1.46)
b2041 (rfbB)	-0.3
b3788 (rffG)	+1.1
dTDP-4-dehydrorhamnose 3,5-epimerase: dTDP-4-dehydro-6-deoxy-α-D-glucopyranose→dTDP-4-dehydro-β-L-rhamnose (EC 5.1.3.13)
b2038 (rfbC)	-0.1
dTDP-4-dehydro-β-L-rhamnose + S-adenosyl-L-methionine→dTDP-4-dehydro-3-methyl-β-L-rhamnose + S-adenosyl-L-homocysteine + H+
No genes
dTDP-4-dehydro-3-methyl-β-L-rhamnose + L-glutamate→dTDP-4-amino-4-deoxy-3-methyl-β-L-rhamnose + 2-oxoglutarate
No genes
dTDP-4-amino-4-deoxy-3-methyl-β-L-rhamnose + S-adenosyl-L-methionine→dTDP-sibirosamine + S-adenosyl-L-homocysteine + H+
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information