MetaCyc Pathway: superpathway of enterobacterial common antigen biosynthesis in Escherichia coli BW25113

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Reactions and Genes LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
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Glucose-1-phosphate thymidylyltransferase:
α-D-glucopyranose 1-phosphate + H+ + dTTP→dTDP-α-D-glucose + diphosphate (EC 2.7.7.24)
b0363 (yaiP) -0.0
b2039 (rfbA) +0.4
b3789 (rffH) +0.7
UDP-N-acetyl-α-D-glucosamine + di-trans,octa-cis-undecaprenyl phosphate→α-D-GlcNAc-PP-Und + UMP (EC 2.7.8.33)
b3784 (rfe) +2.9
UDP-N-acetyl-α-D-mannosamine + 2 NAD+ + H2O→UDP-N-acetyl-α-D-mannosaminuronate + 2 NADH + 3 H+ (EC 1.1.1.336)
b3787 (rffD) +3.0
dTDP-glucose 4,6-dehydratase:
dTDP-α-D-glucose→dTDP-4-dehydro-6-deoxy-α-D-glucopyranose + H2O (EC 4.2.1.46)
b2041 (rfbB) -0.3
b3788 (rffG) +1.1
Lipopolysaccharide N-acetylmannosaminouronosyltransferase:
α-D-GlcNAc-PP-Und + UDP-N-acetyl-α-D-mannosaminuronate→β-D-ManNAcA-(1→4)-α-D-GlcNAc-PP-Und + H+ + UDP (EC 2.4.1.180)
b3794 (rffM) +3.2
dTDP-4-amino-4,6-dideoxygalactose transaminase (in reverse):
dTDP-4-dehydro-6-deoxy-α-D-glucopyranose + L-glutamate→dTDP-4-amino-4,6-dideoxy-α-D-galactose + 2-oxoglutarate (EC 2.6.1.59)
b3791 (rffA) +2.6
dTDP-4-amino-4,6-dideoxy-α-D-galactose + acetyl-CoA→dTDP-4-acetamido-α-D-fucose + coenzyme A + H+ (EC 2.3.1.210)
b3790 (rffC) +3.0
β-D-ManNAcA-(1→4)-α-D-GlcNAc-PP-Und + dTDP-4-acetamido-α-D-fucose→α-D-Fuc4NAc-(1→4)-β-D-ManNAcA-(1→4)-GlcNAc-PP-Und + H+ + dTDP (EC 2.4.1.325)
b4481 (rffT) +2.8
α-D-Fuc4NAc-(1→4)-β-D-ManNAcA-(1→4)-GlcNAc-PP-Und[in] + H+[out]→α-D-Fuc4NAc-(1→4)-β-D-ManNAcA-(1→4)-GlcNAc-PP-Und[out] + H+[in] (EC 7.5.99.a)
b3792 (wzxE) -2.7
n α-D-Fuc4NAc-(1→4)-β-D-ManNAcA-(1→4)-GlcNAc-PP-Und→enterobacterial common antigen + n-1 H+ + n-1 di-trans,octa-cis-undecaprenyl diphosphate
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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information