MetaCyc Pathway: O-antigen building blocks biosynthesis (E. coli) in Escherichia coli BW25113

Add experiment(s):


Reactions and Genes LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
remove
UDP-galactopyranose mutase:
UDP-α-D-galactose→UDP-α-D-galactofuranose (EC 5.4.99.9)
b2036 (glf) +0.1
Glucose-6-phosphate isomerase:
α-D-glucose 6-phosphate→β-D-fructofuranose 6-phosphate (EC 5.3.1.9)
b2843 (kduI) +0.1
b4025 (pgi) -3.4
Phosphoglucomutase (in reverse):
D-glucopyranose 6-phosphate→α-D-glucopyranose 1-phosphate (EC 5.4.2.2)
b0688 (pgm) +2.3
b3176 (glmM) N.D.
Glucose-1-phosphate thymidylyltransferase:
α-D-glucopyranose 1-phosphate + H+ + dTTP→dTDP-α-D-glucose + diphosphate (EC 2.7.7.24)
b0363 (yaiP) -0.0
b2039 (rfbA) +0.4
b3789 (rffH) +0.7
Glutamine--fructose-6-phosphate transaminase (isomerizing):
β-D-fructofuranose 6-phosphate + L-glutamine→D-glucosamine 6-phosphate + L-glutamate (EC 2.6.1.16)
b3729 (glmS) N.D.
Phosphoglucosamine mutase:
D-glucosamine 6-phosphate→α-D-glucosamine 1-phosphate (EC 5.4.2.10)
b3176 (glmM) N.D.
dTDP-glucose 4,6-dehydratase:
dTDP-α-D-glucose→dTDP-4-dehydro-6-deoxy-α-D-glucopyranose + H2O (EC 4.2.1.46)
b2041 (rfbB) -0.3
b3788 (rffG) +1.1
Glucosamine-1-phosphate N-acetyltransferase:
α-D-glucosamine 1-phosphate + acetyl-CoA→N-acetyl-α-D-glucosamine 1-phosphate + coenzyme A + H+ (EC 2.3.1.157)
b3730 (glmU) N.D.
dTDP-4-dehydrorhamnose 3,5-epimerase:
dTDP-4-dehydro-6-deoxy-α-D-glucopyranose→dTDP-4-dehydro-β-L-rhamnose (EC 5.1.3.13)
b2038 (rfbC) -0.1
dTDP-4-dehydrorhamnose reductase (in reverse):
dTDP-4-dehydro-β-L-rhamnose + NADPH + H+→dTDP-β-L-rhamnose + NADP+ (EC 1.1.1.133)
b2040 (rfbD) -0.0
UDP-N-acetylglucosamine diphosphorylase:
N-acetyl-α-D-glucosamine 1-phosphate + H+ + UTP→UDP-N-acetyl-α-D-glucosamine + diphosphate (EC 2.7.7.23)
b3730 (glmU) N.D.

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information