MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Escherichia coli BW25113
| Reactions and Genes | LB Anaerobic with Spiramycin 0.000125 mM |
|---|---|
| remove | |
| Acetolactate synthase: 2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6) | |
| b0078 (ilvH) | -0.2 |
| b3670 (ilvN) | -0.2 |
| b3671 (ilvB) | +0.1 |
| b3769 (ilvM) | -0.2 |
| b0077 (ilvI) | +0.0 |
| Ketol-acid reductoisomerase (in reverse): (S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86) | |
| b3774 (ilvC) | +0.1 |
| Dihydroxy-acid dehydratase: (2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9) | |
| b0269 (yagF) | +0.3 |
| b4297 (yjhG) | +0.0 |
| b3771 (ilvD) | +0.1 |
| Branched-chain-2-oxoacid decarboxylase: 3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72) | |
| No genes | |
| Alcohol dehydrogenase (in reverse): isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1) | |
| b0325 (yahK) | +0.1 |
| b0356 (frmA) | +0.0 |
| b1241 (adhE) | -1.3 |
| b1478 (adhP) | +0.6 |
| b3253 (yhdH) | -1.3 |
| b4269 (yjgB) | +0.3 |
| b3589 (yiaY) | +0.2 |
Links:
- Pathway details at MetaCyc
- Fitness data for 16 genes
- All MetaCyc pathways for Escherichia coli BW25113
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information