MetaCyc Pathway: peptidoglycan biosynthesis V (β-lactam resistance) in Escherichia coli BW25113

Reactions and Genes	LB Anaerobic with Penicillin g 0.0000625 mM
D-alanine--D-alanine ligase: 2 D-alanine + ATP→D-alanyl-D-alanine + ADP + H+ + phosphate (EC 6.3.2.4)
b0092 (ddlB)	-0.2
b0381 (ddlA)	-3.0
Glutamate racemase: L-glutamate→D-glutamate (EC 5.1.1.3)
b3967 (murI)	N.D.
UDP-N-acetylglucosamine 1-carboxyvinyltransferase: UDP-N-acetyl-α-D-glucosamine + phosphoenolpyruvate→UDP-N-acetyl-α-D-glucosamine-enolpyruvate + phosphate (EC 2.5.1.7)
b3189 (murA)	N.D.
UDP-N-acetyl-α-D-glucosamine-enolpyruvate + NADPH + H+→UDP-N-acetyl-α-D-muramate + NADP+ (EC 1.3.1.98)
b3972 (murB)	N.D.
UDP-N-acetylmuramate--L-alanine ligase: UDP-N-acetyl-α-D-muramate + L-alanine + ATP→UDP-N-acetyl-α-D-muramoyl-L-alanine + ADP + H+ + phosphate (EC 6.3.2.8)
b0091 (murC)	N.D.
UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase: UDP-N-acetyl-α-D-muramoyl-L-alanine + D-glutamate + ATP→UDP-N-acetyl-α-D-muramoyl-L-alanyl-D-glutamate + ADP + H+ + phosphate (EC 6.3.2.9)
b0088 (murD)	-0.7
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-lysine ligase: UDP-N-acetyl-α-D-muramoyl-L-alanyl-D-glutamate + L-lysine + ATP→UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysine + ADP + H+ + phosphate (EC 6.3.2.7)
No genes
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase: UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysine + D-alanyl-D-alanine + ATP→UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysyl-D-alanyl-D-alanine + ADP + H+ + phosphate (EC 6.3.2.10)
b0086 (murF)	N.D.
Zinc D-Ala-D-Ala carboxypeptidase: UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysyl-D-alanyl-D-alanine + H2O→D-alanine + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysyl-D-alanine (EC 3.4.17.14)
No genes
Phospho-N-acetylmuramoyl-pentapeptide-transferase: UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysyl-D-alanine + di-trans,octa-cis-undecaprenyl phosphate→Und-PP-MurNAc-L-Ala-γ-D-Glu-L-Lys-D-Ala + UMP (EC 2.7.8.13)
b0087 (mraY)	N.D.
acetylglucosaminyltransferase: Und-PP-MurNAc-L-Ala-γ-D-Glu-L-Lys-D-Ala + UDP-N-acetyl-α-D-glucosamine→Und-PP-β-D-GlcNAc-(1→4)-MurNAc-L-Ala-γ-D-Glu-L-Lys-D-Ala + H+ + UDP (EC 2.4.1.227)
b0090 (murG)	N.D.
Und-PP-β-D-GlcNAc-(1→4)-MurNAc-L-Ala-γ-D-Glu-L-Lys-D-Ala + ammonium→Und-PP-β-D-GlcNAc-(1→4)-MurNAc-L-Ala-γ-D-iGln-L-Lys-D-Ala + H2O
No genes
D-aspartate ligase: Und-PP-β-D-GlcNAc-(1→4)-MurNAc-L-Ala-γ-D-iGln-L-Lys-D-Ala + ATP + D-aspartate→Und-PP-[β-D-GlcNAc-(1→4)]-MurNAc-L-Ala-γ-D-iGln-(6-N-β-D-Asp)-L-Lys-D-Ala + ADP + H+ + phosphate (EC 6.3.1.12)
No genes
Und-PP-[β-D-GlcNAc-(1→4)]-MurNAc-L-Ala-γ-D-iGln-(6-N-β-D-Asp)-L-Lys-D-Ala + ammonium→Und-PP-[β-D-GlcNAc-(1→4)]-MurNAc-L-Ala-γ-D-iGln-(6-N-β-D-Asn)-L-Lys-D-Ala + H2O
No genes
Peptidoglycan glycosyltransferase: 2 Und-PP-[β-D-GlcNAc-(1→4)]-MurNAc-L-Ala-γ-D-iGln-(6-N-β-D-Asn)-L-Lys-D-Ala→a nascent peptidoglycan dimer (E. faecium, tetrapeptide) + H+ + di-trans,octa-cis-undecaprenyl diphosphate (EC 2.4.1.129)
b0084 (ftsI)	N.D.
b0149 (mrcB)	-4.8
b3396 (mrcA)	-0.4
Peptidoglycan glycosyltransferase: Und-PP-[β-D-GlcNAc-(1→4)]-MurNAc-L-Ala-γ-D-iGln-(6-N-β-D-Asn)-L-Lys-D-Ala + a nascent peptidoglycan (E. faecium, tetrapeptide)→a nascent peptidoglycan (E. faecium, tetrapeptide) + di-trans,octa-cis-undecaprenyl diphosphate (EC 2.4.1.129)
b0084 (ftsI)	N.D.
b0149 (mrcB)	-4.8
b3396 (mrcA)	-0.4
a nascent peptidoglycan (E. faecium, tetrapeptide) + a peptidoglycan internal segment (E. faecium, tetrapeptide) + H+→D-alanine + a peptidoglycan tetramer with an L,D cross-link (Enterococcus faecium)
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information