MetaCyc Pathway: long chain fatty acid ester synthesis (engineered) in Escherichia coli BW25113

Reactions and Genes	LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
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Pyruvate decarboxylase: pyruvate + H⁺→acetaldehyde + CO₂ (EC 4.1.1.1)
No genes
Long-chain-fatty-acid--CoA ligase: a long-chain fatty acid + ATP + coenzyme A→a long-chain fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.3)
b1805 (fadD)	+1.3
acetaldehyde + a ubiquinol→ethanol + a ubiquinone (EC 1.1.5.5)
No genes
Long-chain-alcohol O-fatty-acyltransferase: ethanol + a long-chain fatty acyl-CoA→a long-chain acyl ethyl ester + coenzyme A (EC 2.3.1.75)
No genes

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Pathway details at MetaCyc
Fitness data for 1 genes
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All MetaCyc pathways for Escherichia coli BW25113

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information