MetaCyc Pathway: 3-methylbutanol biosynthesis (engineered) in Agrobacterium fabrum C58

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Reactions and Genes D-(+)-melezitose (C)
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2-isopropylmalate synthase:
3-methyl-2-oxobutanoate + acetyl-CoA + H2O→(2S)-2-isopropylmalate + coenzyme A + H+ (EC 2.3.3.13)
Atu2264 -4.2
3-isopropylmalate dehydratase (in reverse):
(2S)-2-isopropylmalate→2-isopropylmaleate + H2O (EC 4.2.1.33)
Atu2709 -4.8
Atu2790 -3.5
3-isopropylmalate dehydratase (in reverse):
2-isopropylmaleate + H2O→(2R,3S)-3-isopropylmalate (EC 4.2.1.33)
Atu2709 -4.8
Atu2790 -3.5
3-isopropylmalate dehydrogenase:
(2R,3S)-3-isopropylmalate + NAD+→(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ (EC 1.1.1.85)
Atu2791 -5.8
3-isopropylmalate dehydrogenase:
(2S)-2-isopropyl-3-oxosuccinate + H+→4-methyl-2-oxopentanoate + CO2 (spontaneous) (EC 1.1.1.85)
Atu2791 -5.8
Branched-chain-2-oxoacid decarboxylase:
4-methyl-2-oxopentanoate + H+→3-methylbutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
3-methylbutanal + NADH + H+→3-methylbutanol + NAD+ (EC 1.1.1.1)
Atu0626 -1.1
Atu1557 +0.2
Atu1595 N.D.
Atu1670 +0.1
Atu2022 -0.0
Atu2138 -0.1
Atu2151 -0.1
Atu2528 +0.1
Atu4236 -0.1
Atu4289 +0.1
Atu5074 -0.2
Atu5138 -0.1
Atu5202 +0.1
Atu5240 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information