MetaCyc Pathway: butanol and isobutanol biosynthesis (engineered) in Agrobacterium fabrum C58

Reactions and Genes	Uridine 5-monophosphate (C)
Glycine oxidase: glycine + dioxygen + H2O→glyoxylate + ammonium + hydrogen peroxide (EC 1.4.3.19; 1.4.3.3)
Atu2330	-0.0
Atu2568	+0.2
3-ethylmalate synthase: glyoxylate + butanoyl-CoA + H2O→3-ethylmalate + coenzyme A + H+ (EC 2.3.3.7)
No genes
3-isopropylmalate dehydrogenase: 3-ethylmalate + NAD+→2-oxovalerate + CO2 + NADH (EC 1.1.1.85)
Atu2791	-4.7
2-oxovalerate→3-methyl-2-oxobutanoate
No genes
Pyruvate decarboxylase: 2-oxovalerate + H+→1-butanal + CO2 (EC 4.1.1.1)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes
Branched-chain-2-oxoacid decarboxylase: 3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse): isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
Atu0626	-1.4
Atu1557	+0.1
Atu1595	N.D.
Atu1670	-0.1
Atu2022	-0.0
Atu2138	+0.0
Atu2151	+0.0
Atu2528	+0.1
Atu4236	-0.0
Atu4289	-0.1
Atu5074	-0.2
Atu5138	+0.2
Atu5202	-0.0
Atu5240	N.D.

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information