MetaCyc Pathway: lipoprotein posttranslational modification (Gram-negative bacteria) in Escherichia coli BW25113

Reactions and Genes	LB Aerobic with Ramoplanin 0.000125 mM
a [prolipoprotein]-L-cysteine[in]→a [prolipoprotein]-L-cysteine[membrane]
No genes
a [prolipoprotein]-L-cysteine + a phosphatidylglycerol→a [prolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine + H+ + sn-glycerol 1-phosphate (EC 2.5.1.145)
b2828 (lgt)	N.D.
Signal peptidase II: a [prolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine + H2O→an (S-diacyl-sn-glyceryl)-L-cysteinyl-[apolipoprotein] + a lipoprotein signal peptide (EC 3.4.23.36)
b0027 (lspA)	N.D.
an (S-diacyl-sn-glyceryl)-L-cysteinyl-[apolipoprotein] + a phosphoglyceride→an N-acyl-(S-diacyl-sn-glyceryl)-L-cysteinyl-[lipoprotein] + a 1-lysophospholipid + H+ (EC 2.3.1.269)
b0657 (lnt)	N.D.
a lipoprotein signal peptide + n H2O→n+1 a peptide
b0176 (yaeL)	N.D.
b1766 (sppA)	+1.6
an N-acyl-(S-diacyl-sn-glyceryl)-L-cysteinyl-[lipoprotein][membrane] + ATP[in] + a LolA lipoprotein chaperone[out] + H2O[in]→a LolA-lipoprotein complex[out] + ADP[in] + H+[in] + phosphate[in]
b0495 (ybbA)	+0.3
b1116 (lolC)	N.D.
b1117 (ycfV)	N.D.
b1118 (lolE)	N.D.
b3463 (ftsE)	+0.3
a LolA-lipoprotein complex[in]→an N-acyl-(S-diacyl-sn-glyceryl)-L-cysteinyl-[lipoprotein][membrane] + a LolA lipoprotein chaperone[in]
b1209 (lolB)	N.D.

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information