MetaCyc Pathway: L-isoleucine biosynthesis I (from threonine) in Escherichia coli BW25113

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Reactions and Genes LB Aerobic with Ramoplanin 0.000125 mM
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Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
b3117 (tdcB) -0.1
b3772 (ilvA) -0.0
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
b3117 (tdcB) -0.1
b3772 (ilvA) -0.0
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
b3113 (yhaR) -0.2
b3117 (tdcB) -0.1
b3772 (ilvA) -0.0
b4243 (yjgF) -1.9
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
b0078 (ilvH) +0.0
b3670 (ilvN) -0.4
b3671 (ilvB) -0.1
b3769 (ilvM) -0.2
b0077 (ilvI) +0.1
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
b3774 (ilvC) -0.1
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
b0269 (yagF) +0.3
b4297 (yjhG) +0.1
b3771 (ilvD) -0.2
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
b3770 (ilvE) -0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information