MetaCyc Pathway: mixed acid fermentation in Pseudomonas putida KT2440

Add experiment(s):


Reactions and Genes 3-hydroxypentanoate
remove
Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)
PP_1505 -0.2
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
PP_1362 -0.3
PP_4301 +0.2
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
PP_1649 +0.5
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
PP_0654 -0.0
PP_2835 +0.0
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
No genes
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
No genes
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
PP_4194 N.D.
formate + H+→CO2 + H2
No genes
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
PP_0897 N.D.
PP_0944 +0.1
PP_1755 -0.2
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
PP_0774 +0.2
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
PP_1457 -0.0
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
PP_2112 -0.0
PP_2336 -3.5
PP_2339 N.D.
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
PP_1616 -2.6
PP_2049 -0.1
PP_2426 -0.0
PP_2650 +0.0
PP_2803 +0.1
PP_3839 -0.1
PP_4760 -0.0
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
No genes
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
PP_2112 -0.0
PP_2336 -3.5
PP_2339 N.D.
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→CO2 + 2-oxoglutarate + NADPH (EC 1.1.1.42)
PP_4011 -0.1
PP_4012 N.D.

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information