MetaCyc Pathway: tetrahydrofolate biosynthesis I in Escherichia coli BW25113

Reactions and Genes	LB Aerobic with Sulfamonomethoxine 0.00025 mM
Dihydropteroate synthase: (7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate→7,8-dihydropteroate + diphosphate (EC 2.5.1.15)
b3177 (folP)	N.D.
Dihydrofolate synthase: 7,8-dihydropteroate + ATP + L-glutamate→7,8-dihydrofolate monoglutamate + ADP + H+ + phosphate (EC 6.3.2.12)
b2315 (folC)	N.D.
Dihydrofolate reductase (in reverse): a 7,8-dihydrofolate + NADPH + H+→a tetrahydrofolate + NADP+ (EC 1.5.1.3)
b0048 (folA)	N.D.
b1606 (folM)	+1.3

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information