MetaCyc Pathway: tunicamycin biosynthesis in Escherichia coli BW25113

Reactions and Genes	LB Aerobic with Sulfamonomethoxine 0.00025 mM
Nucleotide diphosphatase: UTP + H2O→UMP + diphosphate + H+ (EC 3.6.1.9)
b3248 (yhdE)	-0.1
UDP-N-acetyl-α-D-glucosamine→UDP-6-deoxy-5,6-ene-N-acetyl-D-glucosamine + H2O (EC 4.2.1.M11)
No genes
5'-nucleotidase: UMP + H2O→uridine + phosphate (EC 3.1.3.5)
b0383 (phoA)	-0.0
b0480 (ushA)	+0.3
b2291 (yfbR)	+0.2
b2744 (surE)	-1.2
b4055 (aphA)	+0.1
b4374 (yjjG)	-0.1
UDP-6-deoxy-5,6-ene-N-acetyl-D-glucosamine→UDP-N-acetylgalactosamine-5,6-ene (EC 5.1.3.M3)
No genes
UDP-N-acetylgalactosamine-5,6-ene + uridine→UDP-N-acetyl-tunicaminyl-uracil (EC 4.1.99.M2)
No genes
UDP-N-acetyl-tunicaminyl-uracil + H2O→N-acetyl-tunicaminyl-uracil + H+ + UDP (EC 3.6.1.M1)
No genes
N-acetyl-tunicaminyl-uracil + UDP-N-acetyl-α-D-glucosamine→N-acetyl-D-glucosaminyl-N-acetyl-tunicaminyl-uracil + H+ + UDP (EC 2.4.1.M16)
No genes
N-acetyl-D-glucosaminyl-N-acetyl-tunicaminyl-uracil + H2O→N-acetyl-D-glucosaminyl-tunicaminyl-uracil + acetate (EC 3.5.1.M4)
No genes
N-acetyl-D-glucosaminyl-tunicaminyl-uracil + an acyl-[acyl-carrier protein]→a tunicamycin + a soluble [acyl-carrier protein] + H+ (EC 2.3.1.M33)
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information