MetaCyc Pathway: L-isoleucine biosynthesis II in Pseudomonas putida KT2440

Reactions and Genes	L-Arginine (C)
pyruvate + acetyl-CoA + H2O→(R)-citramalate + coenzyme A + H+ (EC 2.3.3.21)
No genes
(R)-2-methylmalate dehydratase: (R)-citramalate→citraconate + H2O (EC 4.2.1.35)
PP_1985	-4.5
PP_1986	-6.8
citraconate + H2O→(2R,3S)-3-methylmalate
PP_1986	-6.8
(2R,3S)-3-methylmalate + NAD+→2-oxobutanoate + CO2 + NADH
No genes
Acetolactate synthase: 2-oxobutanoate + pyruvate + H+→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
PP_1157	N.D.
PP_1394	-1.2
PP_3365	-0.1
PP_3723	-0.9
PP_4679	-4.4
PP_4680	-4.7
(S)-2-aceto-2-hydroxybutanoate + NAD(P)H + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NAD(P)+ (EC 1.1.1.383)
No genes
Dihydroxy-acid dehydratase: (R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
PP_5128	-3.6
Branched-chain-amino-acid transaminase (in reverse): (3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
PP_3511	-5.2

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information