Fitness data for PP_5310 in Pseudomonas putida KT2440

junction-specific ATP-dependent DNA helicase
SEED: ATP-dependent DNA helicase RecG (EC 3.6.1.-)
KEGG: ATP-dependent DNA helicase RecG

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 2.2)


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group condition fitness t score  
agar plate interaction control Parafilmed volatile agar plate with no fungus -3.4 -7.6 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -3.0 -5.8 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -2.8 -8.0 compare
agar plate interaction control Parafilmed volatile agar plate with no fungus -2.8 -7.4 compare
stress Formaldehyde 0.03125 vol% -2.7 -7.5 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -2.6 -5.9 compare
micoeukaryotes Tetrahymena sp., Mixed culture-2 -2.6 -7.2 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -2.5 -6.0 compare
nitrogen source nitrogen source Ammonium chloride 20mM -2.5 -5.5 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -2.3 -4.7 compare
carbon source 3-methyl-1-butanol (C) -2.3 -6.5 compare
carbon source Trisodium citrate (C) -2.3 -5.4 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -2.3 -6.8 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -2.3 -6.8 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -2.2 -6.3 compare
nitrogen source nitrogen source nitrite 0.5mM -2.2 -4.9 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -2.2 -6.1 compare
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. +2.2 +5.3 compare
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. +2.4 +3.9 compare
carbon source 3-Hydroxydecanoic (C) +2.5 +5.1 compare