Fitness data for PP_4748 in Pseudomonas putida KT2440

Amino acid ABC transporter, periplasmic amino acid-binding protein
SEED: Lysine-arginine-ornithine-binding periplasmic protein precursor (TC 3.A.1.3.1)

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.5)


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group condition fitness t score  
phage P. putida Sci small MOI 0.1 -2.0 -3.1 compare
pH pH 7 -0.8 -3.3 compare
pH Growth at pH6 and (C) Trisodium citrate -0.7 -1.5 compare
phage P. putida Missley MOI 0.1 -0.6 -2.3 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.5 -1.3 compare
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.5 -2.2 compare
carbon source butyrate (C) -0.5 -2.2 compare
carbon source carbon source 4-vinylphenol 0.1 mM -0.5 -1.6 compare
phage P. putida Sci Big MOI 0.1 -0.5 -1.3 compare
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.5 -2.1 compare
carbon source carbon source D-Glucose 55.5 mM -0.5 -2.2 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.5 +1.5 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs +0.5 +2.6 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.5 +1.7 compare
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.5 +1.1 compare
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. +0.6 +2.9 compare
stress R2A with Polymyxin B sulfate 0.001 mg/ml +0.6 +2.0 compare
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.6 +1.9 compare
carbon source ferulate (C); with MOPS +0.7 +2.1 compare
stress R2A with Fusaric 240 ug/mL +0.7 +3.5 compare