Fitness data for PP_4669 in Pseudomonas putida KT2440

OmpA family protein
SEED: Integral membrane protein YfiB

Top 20 experiments with the strongest phenotypes (|fitness| ≥ 1.0)

group	condition	fitness	t score
in planta	Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days	-2.7	-2.4	compare
pH	pH 9	-2.2	-2.0	compare
reactor	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.	-2.0	-1.9	compare
soil	Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs	-2.0	-2.7	compare
pH	pH 5	-1.8	-2.0	compare
reactor	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.	-1.7	-1.6	compare
agar plate interaction	Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant	-1.7	-1.5	compare
soil	Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs	-1.5	-1.9	compare
phage	P. putida PUT 7 MOI 1	-1.3	-1.4	compare
nitrogen source	2,6-diaminopimelic (N)	-1.2	-1.7	compare
temperature shift	Temperature shift 30_to_21; with MOPS	-1.1	-1.9	compare
supernatant	Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.2X	-1.1	-2.2	compare
reactor	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.	-1.1	-1.3	compare
carbon source	ferulate (C); with MOPS	-1.1	-1.0	compare
pH	Growth at pH6 and (C) Trisodium citrate	-1.0	-1.4	compare
temperature	Growth at 30C; with MOPS	+1.0	+1.5	compare
carbon source	Tween 20 (C)	+1.0	+1.9	compare
temperature shift	Temperature shift 30_to_25; with MOPS	+1.2	+2.4	compare
pH	Growth at pH6 and (C) D-Glucose	+1.3	+2.8	compare
carbon source	ferulate (C); with MOPS	+1.6	+2.5	compare